中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (6): 340-344.

• 论著 • 上一篇    下一篇

伊曲康唑抑制小鼠树突状细胞迁移及MMP-2、MMP-3、MMP-12与RANTES的分泌

郑晓丽1 梁官钊2 史冬梅3 沈永年2 刘维达2 陈官芝1   

  1. 1.青岛大学附属医院皮肤科，山东青岛，266003 2.中国医学科学院皮肤病医院真菌科，江苏南京，210042 3.济宁市第一人民医院皮肤科，山东济宁，272011
  • 出版日期:2018-06-15 发布日期:2018-12-11
  • 通讯作者: 陈官芝，E-mail：chenguanzhiqd@126.com

Itraconazole inhibits the migration of murine bone marrow derived dendritic cells and secretion of MMP-2, MMP-3, MMP-12 and RANTES

ZHENG Xiaoli1, LIANG Guanzhao2, SHI Dongmei3, SHEN Yongnian2, LIU Weida2, CHEN Guanzhi1   

  1. 1.Department of Dermatology, the Affiliated Hospital of Qingdao University, Qingdao 266003, China; 2.Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China; 3. Department of Dermatology, Jining No. 1 People’s Hospital, Jining 272011, China
  • Online:2018-06-15 Published:2018-12-11
  • Contact: CHEN Guanzhi, E-mail：chenguanzhiqd@126.com

PDF (PC)

123

摘要： 目的：明确伊曲康唑对小鼠骨髓来源树突状细胞（DCs）迁移功能及基质金属蛋白酶(MMP)-2、MMP-3、MMP-12与趋化因子RANTES分泌的影响。方法：取小鼠骨髓单核细胞，重组小鼠粒—巨噬细胞集落刺激因子(rmGM-CSF)诱导至第8天，将DCs分为对照组、伊曲康唑处理组（0.25、0.5、1 μM），细胞计数试剂盒（CCK-8）和Transwell分别检测不同浓度伊曲康唑对DCs活性及DCs迁移情况；Luminex液相芯片技术检测MMP-2、MMP-3、MMP-8、 MMP-12、CCL5/RANTES的分泌；流式细胞仪检测MHCII、CD80、CD86及CCR7的表达。结果：伊曲康唑对树突状细胞毒性呈时间及剂量依赖；伊曲康唑组细胞迁移率低于对照组，差异有统计学意义（P<0.05）。伊曲康唑组MMP-2、MMP-3、MMP-12和RANTES水平均低于对照组，差异均有统计学意义（均P<0.05）。伊曲康唑组和对照组中CCR7和MMP-8水平比较，差异均无统计学意义（P>0.05）。结论：伊曲康可唑抑制树突状细胞的迁移及相关MMPs和RANTES的表达。

关键词: 伊曲康唑, 小鼠树突状细胞, 迁移, MMPs, RANTES

Abstract: Objective: To determine the effects of itraconazole on the migration of murine bone marrow derived dendritic cells (DCs) and the secretion of MMPs and RANTES. Methods: DCs were cultivated for 8 days with rmGM-CSF. DCs were divided into the control group and itraconazole (0.25, 0.5, 1 μM ) group. The viability and migration of DCs were determined by CCK-8 assay and transwell assay respectively. The levels of MMP-2, MMP-3, MMP-8, MMP-12 and RANTES were detected by Luminex. The levels of MHCII, CD80, CD86 and CCR7 were measured by flow cytometry. Results: The cytotoxicity of itraconazole on DCs was time- and dose-dependent. The migration rate and the levels of MMP-2, MMP-3, MMP-12 and RANTES in the itraconazole group were lower than those in the control group (Ps<0.05). There was no significant difference in the level of CCR and MMP-8 between the two groups (P>0.05). Conclusion: Itraconazole can inhibit the DCs cell migration and secretion of MMPs and RANTES.

Key words: itraconazole, DCs, migration, MMPs, RANTES