清热利湿饮,Cyclin E,泛素化,FBW7 ," /> 清热利湿饮,Cyclin E,泛素化,FBW7 ,"/> Qingrelishi recipe,Cyclin E,ubiquitination,FBW7 ,"/> <p class="MsoNormal"> <span>清热利湿饮通过调控</span>FBW7<span>影响</span><span>HaCaT</span><span>细胞</span><span>Cyclin E</span><span>的表达</span>

中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (3): 149-152.

• 论著 • 上一篇    下一篇

清热利湿饮通过调控FBW7影响HaCaT细胞Cyclin E的表达

孙淑娜1  黄启腾1  魏海峰2  李广远3  赵颖1  张晓杰1   

  1. 1山东中医药大学附属医院皮肤科，济南,250011

    2山东中医药大学，济南,250355

    3中国农业银行山东分行，济南,250014

  • 出版日期:2017-03-15 发布日期:2018-12-14
  • 通讯作者: 张晓杰, E-mail: qlzxj@126.com

The expression of Cyclin E affected by Qingrelishi recipe through regulating FBW7 in HaCaT cells

SUN Shuna1, HUANG Qiteng1, WEI Haifeng2, LI Guangyuan3, ZHAO Ying1, ZHANG Xiaojie1   

  1. 1. Department of Dermatology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250012, China; 2. Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 3. Agricultural Bank of China Shandong Branch, Jinan 250014, China

  • Online:2017-03-15 Published:2018-12-14
  • Contact: ZHANG Xiaojie, E-mail: qlzxj@126.com

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摘要：

目的：明确清热利湿饮调控HaCaT细胞内Cyclin E表达的机制。方法：Western blot检测HaCaT细胞蛋白半衰期；免疫沉淀及Western blot检测蛋白泛素化水平；Western blot及Real-time PCR检测清热利湿饮对E3泛素连接酶SCF-FBW7表达的影响。结果：与对照组比较,清热利湿饮组Cyclin E蛋白的半衰期较对照组缩短，Cyclin E蛋白泛素化水平及蛋白降解速度显著增高，FBW7 mRNA与蛋白水平明显上调。结论：清热利湿饮通过调控FBW7抑制Cyclin E的表达

关键词: 清热利湿饮')">

清热利湿饮, Cyclin E, 泛素化, FBW7

Abstract:

Objective: To determine the mechanism of the expression of Cyclin E  affected by Qingrelishi recipe in HaCaT cells. Methods: Half-life of Cyclin E in HaCaT cells was detected by Western blot. The ubiquitination level of Cyclin E was detected through IP and Western blot. The level of ubiquitin ligase E3 SCF-FBW7 affected by Qingrelishi recipe was detected by Western blot and Real-time PCR. Results: Compared with control group, the Half-life of Cyclin E was shorter, the ubiquitination level and the degradation speed of Cyclin E were higher and the level of FBW7 mRNA and protein was higher in the Qingrelishi recipe group. Conclusion: Qingrelishi recipe can inhibit the Cyclin E expression through the regulation of FBW7

Key words: Qingrelishi recipe')">

Qingrelishi recipe, Cyclin E, ubiquitination, FBW7