申克孢子丝菌,单核细胞白血病,肿瘤坏死因子α,NF-κB ," /> 申克孢子丝菌,单核细胞白血病,肿瘤坏死因子α,NF-κB ,"/> sporothrix schenckii,monocytic leukemia,TNF-α,NF-κB ,"/> <p class="MsoNormal"> Effects of <span>〖</span><span>WTHX</span><span>〗</span><span>Sporothrix schenckii</span><span>〖</span><span>WTHZ</span><span>〗 </span><span>yeasts on the activation of NF-</span><span>κ</span><span>B signal pathway and secretion of TNF-</span><span>α </span><span>in human acute monocytic leukemia cells</span>

China Journal of Leprosy and Skin Diseases ›› 2017, Vol. 33 ›› Issue (2): 74-78.

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Effects of 〖WTHX〗Sporothrix schenckii〖WTHZ〗 yeasts on the activation of NF-κB signal pathway and secretion of TNF-α in human acute monocytic leukemia cells

LIU Caixia1, DUAN Zhimin1, DU Leilei1, ZENG Rong1, SHEN Yongnian1, HU Suquan1, LIU Weida1, CHEN Qing2, LI Min1.   

  1. 1. Institute of Dermatology, Chinese Academy of Medical Sicences and Peking Union Medical College, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases, Nanjing 210042, China; 2. Jiangsu Province Blood Center, Nanjing 210042, China

  • Online:2017-02-15 Published:2018-12-13
  • Contact: LI Min, E-mail: drlimin@sina.cn CHEN Qing, E-mail: qngchen@hotmail.com

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Abstract:

Objective: To determine the effects of Sporothrix schenckii yeasts on the activation of NF -κB signal pathway and secretion of TNF-α in human acute monocytic leukemia cells (THP-1). Methods: The expression of TNF-α mRNA was detected by Real-time fluorescence quantitative PCR and enzyme-linked immunosorbent assay respectively. The level of phosphorylated IκBα was detected by Western blot. NF-κB-p65 nuclear translocation was measured by immunofluorescence. The level of TNF-α mRNA in THP-1 pretreated with 100 nM Dexamethasone (a NF-κB inhibitor) for 30 minutes was detected by Real-time fluorescence quantitative PCR. Results: The levels of TNF-α mRNA in THP-1 cells treated with Sporothrix schenckii yeasts for 6 hours were increased compared with the blank control group (P<0.001). The secretion level of TNF-α in the Sporothrix schenckii yeasts group was 4610.419±121.501 pg/mL, which was higher than that in the blank group (186.964±98.073 pg/mL), with a significant difference (P<0.001). Phosphorylation IκBα protein increased obviously and in a time -dependent manner after treated with Sporothrix schenckii yeasts from 30 minutes to 60 minutes. The fluorescent intensity of NF-κB-p65 in the Sporothrix schenckii yeasts group was stronger than that in the blank group. The level of TNF-α mRNA was decreased in the THP-1 macrophages treated with 100 nM dexamethasone in the three groups. Conclusion: Sporothrix schenckii yeasts can increase the expression of TNF-α through enhancing the activation of NF-κB pathway.

Key words: sporothrix schenckii')">

sporothrix schenckii, monocytic leukemia, TNF-α, NF-κB