cured patients with leprosy,IL-10,CD4+CD25+Foxp3+Treg ,"/> 麻风康复者外周血Treg细胞及IL-10水平检测

中国麻风皮肤病杂志 ›› 2019, Vol. 35 ›› Issue (3): 158-160.doi: 10.12144/zgmfskin201903158

• 短篇论著 • 上一篇    下一篇

麻风康复者外周血Treg细胞及IL-10水平检测

丘文苑1  林笛1  林悦芹1   谢彩霞1  蓝菲莹1  谢龙凤1  罗权2  张锡宝2   

  1. 1广东省泗安医院,东莞,523000
    2广州市皮肤病防治所,广州,510095
  • 出版日期:2019-03-15 发布日期:2019-04-08
  • 通讯作者: 张锡宝,E-mail:zxibao@126.com

Detection of Treg Cells and IL-10 in the Peripheral Blood of the Cured Patients with Leprosy

QIU Wenyuan1, LIN Di1, LIN Yueqin1, XIE Caixia1, LAN Feiying1, XIE Longfeng1, LUO Quan2, ZHANG Xibao2   

  1. 1. Guangdong Province Sian Hospital, Dongguan 52300, Guangdong, China; 2. Department of the Skin Laser Guangzhou Institute of Dermatology, Guangzhou 510095, Guangdong, China
  • Online:2019-03-15 Published:2019-04-08
  • Contact: ZHANG Xibao, E-mail: zxibao@126.com

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摘要： 目的：通过检测麻风康复者外周血中CD4+CD25+Foxp3+Treg细胞百分比、IL-10的表达水平,探讨麻风康复者的细胞免疫水平。方法：采用流式细胞技术检测25例麻风康复者和20名健康者对照的外周血中CD4+CD25+Foxp3+Treg细胞的水平变化,ELISA法检测血清中IL-10水平,并进行相关性分析。 结果：麻风康复者组外周血CD4+CD25+Foxp3+Treg细胞占CD4+CD25+Treg淋巴细胞的比率和血清IL-10浓度分别为(7.45±1.43)%和(13.45±3.61)pg/mL,均高于正常对照组的(5.89±1.21)%和(9.23±1.51)pg/mL（P＜0.01）。麻风康复者及正常对照外周血CD4+CD25+Foxp3+Treg细胞水平与血清中IL-10含量呈正相关（P＜0.05）。结论：麻风康复者外周血中CD4+CD25+Foxp3+Treg细胞与血清中IL-10高于正常对照,麻风康复者可能存在细胞免疫失调状态。

关键词: 麻风康复者, IL-10, CD4+CD25+Foxp3+Treg细胞

Abstract: Objective: To detect the level of CD4+CD25+Foxp3+Treg cells and interleulin-10 (IL-10) in the peripheral blood from the cured patients with leprosy in order to explore the mimmune status of them. Methods: The levels of CD4+CD25+Foxp3+Treg in T lymphocyte subgroups and IL-10 in peripheral blood were detected by flow cytometry and ELISA respectilvely in 25 cured patients with leprosy and 20 healthy controls. Results: The level  CD4+CD25+Foxp3+Treg in T lymphocytes and the level of IL-10 in the patients were (7.45±1.43)% and (13.45±3.61) pg/mL, which were higher than those in controls (5.89±1.21)% and (9.23±1.51) pg/mL, with significant differences (P<0.01). There was a significant positive correlation between  CD4+CD25+Treg cells and IL-10 in the cured patients and healthy controls. Conclusion: The level of CD4+CD25+Foxp3+Treg cells and IL-10 in blood serum in the cured patients with leprosy were higher than those in healthy controls, which indicated the cured patients with leprosy may have Immune imbalance.

Key words: cured patients with leprosy')">

cured patients with leprosy, IL-10, CD4+CD25+Foxp3+Treg