中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (8): 467-472.doi: 10.12144/zgmfskin202008467

• 论著 • 上一篇    下一篇

长链非编码RNA在Kaposi肉瘤组织中的表达

刘治全1,袁虎2,普雄明3   

  1. 1石河子大学医学院，石河子，832002;2湖南省中医药研究院附属医院，长沙，410006;3新疆维吾尔自治区人民医院，乌鲁木齐,830001
  • 出版日期:2020-08-15 发布日期:2020-08-11
  • 通讯作者: 普雄明，E-mail: puxiongming@126.com

Expression of long non-coding RNA in Kaposi's sarcoma

LIU Zhiquan1, YUAN Hu2, PU Xiongming3   

  1. 1 Medical College of Shihezi University, Shihezi 832002, China; 
    2 Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine, Changsha 410006,China; 
    3 People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
  • Online:2020-08-15 Published:2020-08-11
  • Contact: PU Xiongming, E-mail: puxiongming@126.com

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摘要： 目的：检测长链非编码RNA(lncRNA)在Kaposi肉瘤组织和瘤旁正常组织中的表达。方法：利用高通量lncRNA芯片技术检测5例Kaposi肉瘤组织及其瘤旁正常组织的lncRNA 表达谱，筛选差异表达的lncRNA，并利用实时荧光定量PCR(qRT-PCR)方法进一步验证芯片结果，同时采用生物信息学分析差异lncRNA靶基因KEGG通路注释。结果：与正常组织相比，Kaposi肉瘤组织中共筛选出717个差异表达的lncRNA，其中上调表达408个，下调表达309个；与正常组织相比，lnc-PXDN-3:3和lnc-PERP-10:2在Kaposi肉瘤组织中均表达上调，与芯片结果一致。生物学过程注释集中在Wnt信号通路、泛素化代谢、TGF-beta信号通路、P53信号通路。结论：lncRNA可能与Kaposi肉瘤发病有关。

关键词: Kaposi肉瘤, 长链非编码RNA, 基因芯片, 实时荧光定量PCR

Abstract: Objective: To detect the differential expression of long non-coding RNA (lncRNA) in Kaposi's sarcoma (KS) tissues and normal adjacent tissues. Methods: The lncRNA expression profiles were detected by high-throughput lncRNA microarray technology in Kaposi's lesions and the adjacent normal tissues in 5 patients. The lncRNAs of differential expression between lesions and adjacent normal tissues were screened, and the results of the microarray were further verified by qRT-PCR. The KEGG pathway annotation of different lncRNA target genes was analyzed by bioinformatics. Results: A total of 717 lncRNAs of differential expression were screened, of which 408 were up-regulated and 309 were down-regulated in Kaposi's sarcoma tissues. The expression of lnc-PXDN-3:3 and lnc-PERP-10:2 in Kaposi's sarcoma tissues was all significantly higher, which was consistent with the chip results. Biological process annotation focuses on Wnt signal pathway, ubiquitin metabolism, TGF-beta signal pathway, p53 signal pathway and so on.Conclusion: lncRNA may be related to Kaposi's sarcoma.

Key words: Kaposi's sarcoma, long non-coding RNA, gene chip, qRT-PCR