中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (9): 533-536.doi: 10.12144/zgmfskin202009533

• 论著 • 上一篇    下一篇

Caspase抑制剂对特应性皮炎患者外周血CD4+T细胞亚群分泌细胞因子的影响

李圆圆1,郝宝珍2,孟云芳1   

  1. 1山东第一医科大学附属省立医院皮肤科，山东济南，250021；
    2山东省妇幼保健院，山东济南，250014
  • 出版日期:2020-09-15 发布日期:2020-08-21
  • 通讯作者: 孟云芳，E-mail: mengyunfang6.12@163.com

Effect of Z-VAD-FMK, a broad caspase inhibitor, on the expression of cytokines released by CD4+T cell subset in the patients with atopic dermatitis

LI Yuanyuan1, HAO Baozhen2, MENG Yunfang1   

  1. 1 Department of Dermatology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China; 2 Shandong Provincial Maternity&Child-care Hospital, Jinan 250014, China
  • Online:2020-09-15 Published:2020-08-21
  • Contact: MENG Yunfang, E-mail: mengyunfang6.12@163.com

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摘要： 目的：明确广谱半胱氨酸天冬氨酸蛋白酶(caspase)抑制剂对特应性皮炎(AD)患者外周血CD4+T细胞亚群分泌细胞因子的影响。方法：广谱caspase抑制剂Z-VAD-FMK与30例AD患者外周血单一核细胞(PBMCs)体外共培养后,PBMCs分为3组，分别加入Z-VAD-FMK溶液、地塞米松溶液和PBS溶液。流式细胞仪检测CD4+T细胞亚群；ELISA方法检测上清液中IFN-γ、IL-4、IL-17的浓度。结果：IFN-γ、IL-4、IL-17水平在PBS溶液组高于Z-VAD-FMK组，差异均有统计学意义(均P<0.01)，在Z-VAD-FMK组与地塞米松组间比较，均无统计学意义(均P>0.05)。结论：广谱caspase抑制剂Z-VAD-FMK可抑制AD患者PBMC中CD4+T细胞Th1、Th2、Th17分泌相关细胞因子。

关键词: 皮炎, 特应性；半胱氨酸天冬氨酸蛋白酶；CD4+T细胞

Abstract: Objective: To determine the effect of Z-VAD-FMK, a broad caspase inhibitor, on the expression of cytokines released by CD4+T cells subset in the patients with atopic dermatitis (AD). Methods: PBMCs from 30 AD patients were co-cultured with Z-VAD-FMK solution in vitro, then the cells were divided into PBS group, Z-VAD-FMK group and dexamethasone group. The levels of CD4+T cells were detected by flow cytometry. The level of IFN-γ, IL-4 and IL-17 were detected by ELISA. Results: The levels of IFN-γ, IL-4 and IL-17 in the PBS group were higher than that in the Z-VAD-FMK group (Ps<0.01) and there was no significant differences of these between the Z-VAD-FMK group and dexamethasone group (Ps>0.05). Conclusion: Z-VAD-FMK can inhibit the expression of cytokines released by CD4+T cells subset of Th1, Th2 and Th17 in patients with AD.

Key words: dermatitis, atopic, caspases, CD4+T cells