中国麻风皮肤病杂志 ›› 2021, Vol. 37 ›› Issue (3): 143-147.doi: 10.12144/zgmfskin202103143

• 论著 • 上一篇    下一篇

丹参酮治疗雄激素性秃发大鼠模型的研究

胡嘉元1,李锘2,底君3,王思丹4,刘青武5,6,陈静7,杨顶权2   

  1. 1首都医科大学附属北京中医医院皮肤科，北京，100010；2中日友好医院皮肤与性病科，北京，100029；3太阳宫社区卫生服务中心中医皮肤科，北京，100028；4钟祥市中医院皮肤科，湖北钟祥，431900；5北京中医药大学，北京，100029；6首都医科大学附属北京中医医院北京市中医研究所，北京，100010；7浙江省皮肤病医院，浙江德清，313200
  • 出版日期:2021-03-15 发布日期:2021-03-03
  • 通讯作者: 杨顶权，E-mail: ydqlx@163.com

Study on the therapeutic effect of Danshinone on androgenetic alopecia rat model

HU Jiayuan1, LI Nuo2, DI Jun3, WANG Sidan4, LIU Qingwu5,6, CHEN Jing7, YANG Dingquan2   

  1. 1 Department of Dermatology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010, China; 2 Department of Dermatology, China Japan Friendship Hospital, Beijing 100029, China; 3 Department of Traditional Chinese Medicine Dermatology, Sun Palace Community Health Service Center, Beijing 100028, China; 4 Department of Dermatology, Zhongxiang Hospital of Traditional Chinese Medicine, Hubei 431900, China; 5 Beijing University of Chinese Medicine, Beijing 100029, China; 6 Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China; 7 Zhejiang Provincial Dermatology Hospital, Deqing 313200, China
  • Online:2021-03-15 Published:2021-03-03
  • Contact: YANG Dingquan, E-mail: ydqlx@163.com

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摘要： 目的：研究丹参酮对二氢睾酮（DHT）诱导的雄激素性秃发（AGA）大鼠模型的治疗作用。方法：32只Wistar雄性大鼠随机分为空白对照组、DHT造模组、丹参酮组及非那雄胺组，每组8只；空白对照组大鼠不予处理，余24只大鼠应用皮下注射5 mg/kg·d DHT的方法建立AGA大鼠模型。DHT造模组大鼠不予干预；丹参酮组大鼠予0.24 g/kg·d丹参酮进行灌胃干预，灌胃从造模的第二天开始，连续给药60 d；非那雄胺组大鼠予0.12 mg/kg·d非那雄胺进行灌胃干预，时间与丹参酮组一致。观察大鼠毛发的形态学改变及皮肤组织病理学改变，检测大鼠血清及组织的雌二醇（E2）、睾酮（T）、DHT、酸性成纤维细胞生长因子（αFGF）、胰岛素样生长因子（IGF-1）、肿瘤坏死因子（TNF-α）、转化生长因子β1（TGF-β1）水平。结果：DHT造模组大鼠背部毛发与空白对照组大鼠相比明显稀疏，部分毛发脱落，血清TNF-α、TGF-β1浓度较空白对照组明显升高（P<0.05）；丹参酮组大鼠与DHT造模组大鼠相比，背部毛发分布整齐，毛发较为浓密；丹参酮组大鼠的血清雌二醇浓度较二氢睾酮造模组显著升高（P<0.05），血清DHT浓度较DHT造模组明显降低（P<0.05）；丹参酮组大鼠组织αFGF的浓度显著高于DHT造模组大鼠（P<0.05）。结论：丹参酮治疗AGA模型大鼠有效，其治疗AGA的机制与调节组织和循环的激素代谢水平及细胞因子作用相关。

关键词: 雄激素性秃发, 大鼠模型, 二氢睾酮, 丹参酮

Abstract: Objective: To evaluate the therapeutic effect of Danshinone on androgenetic alopecia (AGA) rat model induced by dihydrotestosterone (DHT). Methods: Thirty-two Wistar male rats were randomly divided into blank control group, DHT model group, Danshinone group and finasteride group with eight rats in each group. Rats in the blank control group were not treated whilethe other 24 rats were subcutaneously injected with DHT for 5 mg/kg once a day, to establish AGA rat model. Rats in DHT model group were not intervened. Rats in the Danshinone group were given Danshinone  by gavage for 0.24 g/kg once a day for 60 days starting from the second day of modeling. Rats in the finasteride group were given finasteride for 0.12 mg/kg once a day for 60 days. The changes of the hair and histopathology of the model rats were assessed, and the level of hormones and cytokines including estradiol (E2), testosterone, DHT, α fibroblast growth factor (αFGF), insulin-like growth factor 1 (IGF-1), tumor necrosis factor -α (TNF-α),transforming growth factor β1 (TGF-β1) in the serum and tissue of the rats were detected by ELISA. Results: The back hair of the rats in the DHT group were significantly thinner than that of the rats in the blank control group. The levels of TNF-α and TGF-β1 of DHT group were higher than that of blank control group (P<0.05). The back hair on the rats of Danshinone group were denser than that of the DHT group. Compared with the DHT group, the level of serum DHT in the Danshinone group was lower and the level of serum estradiol was higher (P<0.05). The level of αFGF of the tissue in Danshinone group was higher than that in DHT group (P<0.05). Conclusion: Danshinone is effective in treating AGA model rats, and the mechanism may be related to the regulation of hormone metabolism and cytokines.

Key words: androgenetic alopecia, rat model, dihydrotestosterone, Danshinone