中国麻风皮肤病杂志 ›› 2022, Vol. 38 ›› Issue (2): 101-103.doi: 10.12144/zgmfskin202202101

• 论著 • 上一篇    下一篇

免疫荧光技术在皮肤活检组织真菌染色中的应用

陈学超,于永翔,王建文,暴芳芳,周桂芝,陈声利,卢宪梅,刘永霞   

  1. 山东第一医科大学附属皮肤病医院(山东省皮肤病医院)，山东省皮肤病性病防治研究所，济南，250022
  • 出版日期:2022-02-15 发布日期:2021-12-20
  • 通讯作者: 刘永霞，E-mail: liuyx_comeon@163.com

Application of immunofluorescent staining method for detection of fungi in dermatopathology

CHEN Xuechao, YU Yongxiang, WANG Jianwen, BAO Fangfang, ZHOU Guizhi, CHEN Shengli, LU Xianmei, LIU Yongxia   

  1. Shandong Provincial Hospital for Skin Diseases & Shandong Provincial Institute of Dermatology and Venereology, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250022, China
  • Online:2022-02-15 Published:2021-12-20
  • Contact: LIU Yongxia, E-mail: liuyx_comeon@163.com

PDF (PC)

282

摘要： 目的：评价免疫荧光染色法在皮肤活检组织真菌染色中的应用价值。方法：经组织病理和组织培养确诊的43例皮肤真菌病患者对其石蜡包埋切片分别进行免疫荧光染色、PAS染色。结果：43例皮肤真菌病中PAS染色阳性27例（62.8%），免疫荧光染色阳性30例（69.7%），免疫荧光染色法较PAS染色阳性率有所提高，两者差异无统计学意义（P>0.05）。同时两种方法均阴性患者5例，两种方法联合真菌检出阳性率为88.4%。结论：免疫荧光染色是提高皮肤活检组织中真菌检出的重要补充。

关键词: 免疫荧光技术, 皮肤真菌病, 活检组织

Abstract: Objective: To evaluate the application of immunofluorescent staining method in detecting the fungi in skin tissues. Methods: A total of 43 formalin-fixed paraffin-embedded tissues which were indentified as fungal infection by pathology and fungal culture were stained with Calcofluor White and Periodic acid-Schiff （PAS）stain. Results: In 43 cases, 27 cases (62.8%) were positive of PAS, 30 cases (69.7%) were positive of immunofluorescent staining, with no significant difference (P>0.05). There were 5 patients both negitive of immunofluorescent staining and PAS and the positivity rate was 88.4% of two methods combined.  Conclusion: Fluorescent staining is an supplement for detection of fungi in skin tissues.

Key words: immunofluorescent staining, dermatomycosis, biospy