中国麻风皮肤病杂志 ›› 2022, Vol. 38 ›› Issue (10): 673-677.doi: 10.12144/zgmfskin202210673

• 论著 • 上一篇    下一篇

依巴斯汀通过抑制AKT/mTOR通路诱导人黑素瘤细胞自噬

张平1，2,王静3,王颖超1，4,倪莉4,李明明4,党宁宁4   

  1. 1山东大学齐鲁医学院，济南，250012；2山东大学附属济南市中心医院，济南，250013；3滨州医学院附属医院皮肤科，滨州，256600；4山东第一医科大学附属省立医院，济南，250021
  • 出版日期:2022-10-15 发布日期:2022-08-22

Ebastine induces autophagy in human melanoma cells by inhibiting AKT/mTOR pathway

ZHANG Ping1，2, WANG Jing3, WANG Yingchao1，4, NI Li4, LI Mingming4, DANG Ningning4   

  1. 1 Cheeloo College of Medicine, Shandong University, Jinan 250012, China; 2 Jinan Central Hospital, Shandong University, Jinan 250013, China; 3 Department of Dermatology, Binzhou Medical University Hospital, Binzhou 256600, China; 4 Shandong Provincial Hospital, Shandong First Medical University, Jinan 250021, China
  • Online:2022-10-15 Published:2022-08-22

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摘要： 目的：研究依巴斯汀对人黑素瘤细胞自噬的影响及机制。方法：体外培养人黑素瘤细胞A375和M14,采用CCK-8增殖实验检测细胞活力，并计算IC50;利用mCherry-EGFP-LC3B双荧光指示系统检测自噬流；采用Western blot验证自噬相关蛋白LC3,Beclin1及信号通路蛋白的表达。结果：依巴斯汀可显著抑制人黑素瘤细胞的活力；依巴斯汀明显诱导人黑素瘤细胞中自噬小体和自噬溶酶体的产生；依巴斯汀显著上调人黑素瘤细胞中LC3-Ⅱ/Ⅰ的比值以及Beclin1的表达，同时抑制AKT/mTOR信号通路的活化，降低p-AKT/AKT和p-mTOR/mTOR。结论：依巴斯汀通过抑制AKT/mTOR通路诱导人黑素瘤细胞自噬的发生。

关键词: 依巴斯汀, 人黑素瘤细胞, 自噬, AKT/mTOR信号通路

Abstract: Objective: To study the effect and mechanism of ebastine on autophagy of human melanoma cells. Methods: The human melanoma cells A375 and M14 were cultured in vitro and cell viability was detected by a cell counting kit (CCK-8) and IC50 was calculated. The autophagy flow was detected by mCherry-EGFP-LC3B fluorescence plasmid. Western blot was used to detect the expression level of autophagy-related proteins LC3, Beclin1 and signaling pathway proteins. Results: After the action of ebastine, the cell viabilities of human melanoma cells decreased, the production of autophagosomes and autophagolysosomes in human melanoma cells increased, the ratio of LC3-Ⅱ/Ⅰ and the expression of Beclin1 increased, the activation of AKT/mTOR signaling pathway decreased, and p-AKT/AKT and p-mTOR/mTOR in human melanoma cells decreased. Conclusion: Ebastine induces significant autophagy in human melanoma cells by inhibiting the AKT/mTOR signaling pathway.

Key words: ebastine, human melanoma cells, autophagy, AKT/mTOR signaling pathway