中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (1): 14-17.

• 论著 • 上一篇    下一篇

电离辐射对瘢痕疙瘩成纤维细胞增殖的影响

王立 张力元 冀胜军 季江 冷红 苏玉华   

  1. 苏州工业园区斜塘人民医院莲花第一社区卫生服务站，215000
    2苏州大学附属第二医院，苏州，215004
    3南京医科大学附属苏州医院，215002
  • 出版日期:2016-01-15 发布日期:2020-09-15
  • 通讯作者: 季江，E-mail:jijiang2222@126.com

Effects of ionizing radiation on the proliferation of keloid fibroblasts

WANG Li, ZHANG Liyuan, JI Shengjun, JI Jiang, LENG Hong, SU Yuhua   

  1. 1. Xietang People‘s Hospital No.1 Lotus Community Medical Service, Suzhou Industrial Park, Suzhou, 215004; 2. Second Hospital Affiliated to Soochow University, Suzhou 215004, China; 3. Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou 215002, China
  • Online:2016-01-15 Published:2020-09-15
  • Contact: JI Jiang, E-mail:jijiang2222@126.com

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摘要： 目的：明确电离辐射对瘢痕疙瘩成纤维细胞（KFb）增殖的影响。方法：MTT比色法、流式细胞仪检测不同剂量电离辐射照射前后KFb形态、增殖和存活力变化;通过测定培养基上清液中羟脯氨酸含量,明确处理前后胶原总体水平变化。结果：4Gy X-Rays作用72 h后,KFb增殖抑制率为（47.88±1.82）%高于正常皮肤成纤维细胞（39.86±0.07）%（P〈0.05）;KFb细胞的形态发生了明显的变化,KFb细胞培养上清液中羟脯氨酸含量照射72 h后为（11.749±0.9428）μg/mL高于照射前的（18.689±0.8484）μg/mL（P〈0.05）。结论：电离辐射能够抑制KFb细胞的增殖及胶原的产生。

关键词: 电离辐射, 瘢痕疙瘩成纤维细胞, 胶原

Abstract: Objective: To determine the effects of ionizing radiation on the proliferation of keloid fibroblasts (KFb). Methods: The cllular morphology, viability, proliferation and cell cycle of KFb before and after ionizing radiation were detected by MTT and flow cytometry. The change of the collagen level before and after treatment was measured by detecting the level of the hydroxyproline in the supernatant of the co-culture. Results:The proliferation inhibition rate of KFb after 4Gy irradiation for 72 hours was (47.88±1.82)%  which was higher than that of normal skin fibroblast (39.86±0.07)%, (P<0.05). The cellular morphology of KFb was abnormal. The level of hydroxyproline was (11.749±0.9428) μg/mL and (18.689±0.8484)μg/mL before and after irradiation for 72 hours respectively (P<0.05). Conclusion: Ionizing radiation inhibits the proliferation of KFb and the generation of collagen

Key words: ionizing irradiation, keloid fibroblast, collagen