中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (3): 139-142.

• 论著 • 上一篇    下一篇

对称性肢端角化病皮损中PrxI、CLASP1的表达

于作忠 程云龙* 盛 萍 黎世杰 樊翌明   

  1. 广东医学院附属医院皮肤科 湛江，524001
  • 出版日期:2016-03-15 发布日期:2018-12-19
  • 通讯作者: 樊翌明，Email：ymfan1963@163.com *并列第一作者
  • 基金资助:
    广东医学院科技创新基金（STIF201103）

Expression of peroxiredoxin I，cytoplasmic linker associated protein 1 in lesions of symmetrical acrokeratoderma

YU Zuozhong, CHENG Yunlong, SHENG Ping, LI Shijie, FAN Yiming.   

  1. Department of Dermatology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China
  • Online:2016-03-15 Published:2018-12-19
  • Contact: Fan Yi-Ming, E-mail: ymfan1963@163.com

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摘要： 目的： 检测对称性肢端角化病（SAK）皮损中过氧化物还原酶I（Prx I）、胞质链接相关蛋白1（CLASP1）的表达水平。方法：用RT-PCR、免疫组化方法检测9例SAK患者腕部皮损及其周围皮肤和9名正常人腕部皮肤中的Prx I、CLASP1mRNA及蛋白质的表达水平。结果：SAK皮损中Prx I和CLASP1 mRNA水平为（0.94±0.66）和（0.95±0.76），明显高于皮损周围（0.51±0.20,0.56±0.31）和正常皮肤（0.45±0.26,0.47±0.33）。SAK皮损中Prx I阳性细胞弥漫分布于表皮基底层和棘层，而CLASP1阳性染色主要位于棘层，二者的阳性染色范围及程度明显高于皮损周围和正常人皮肤。结论：SAK皮损中Prx I和CLASP1表达上调，可能参与表皮角质形成细胞过度增殖和异常分化。

关键词: 对称性肢端角化病, 过氧化物还原酶I, 胞质链接相关蛋白1

Abstract: Objective：To detect the expression level of peroxiredoxin I (Prx I) and cytoplasmic linker associated protein 1 (CLASP1) in the lesions of symmetrical acrokeratoderma (SAK). Methods: The level of Prx I and CLASP1 mRNA in lesions and perilesional skin from 9 patients and normal skin from 9 healthy controls was detected by RT-PCR and the level of protein was detected by immunohistochemistry. Results: The expression level of Prx I and CLASP1 mRNA in lesions was (0.94±0.66) and (0.95±0.76), which was higher than those in perilesional skin (0.51±0.20 and 0.56±0.31) and normal skin (0.45±0.26 and 0.47±0.33). Positive cells of Prx I were in the stratum spinosum and basal layers predominantly in the skin lesions, while that of CLASP1 were in the stratum spinosum. The number and degree of positive cells of Prx I and CLASP1 in lesions were more than those in the perilesional and normal skin. Conclusion: Overexpression of Prx I and CLASP1 might be involved in the hyperproliferation and abnormal differentiation of epidermal keratinocytes in the lesions of SAK.

Key words: symmetrical acrokeratoderma, peroxiredoxin I, cytoplasmic linker associated protein