中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (7): 397-398.

• 论著 • 上一篇    下一篇

液体培养法、PCR法和SAT法在解脲支原体检测中的应用比较

曾成龙， 冯婷， 闫 丹， 王胜春   

  1. 第四军医大学西京皮肤医院，陕西西安，710032
  • 出版日期:2016-07-15 发布日期:2018-12-27
  • 通讯作者: 王胜春，E-mail：wangsc@fmmu.edu.cn

Comparison of SAT, Liquid culture and PCR in detection of ureaplasma urealyticum

ZENG Chenglong, FENG Ting, YAN Dan, WANG Shengchun   

  1. Army Skin Disease Research Institute, Xijing Hospital, Fourth Military Medical University, Xi´an 710032, China
  • Online:2016-07-15 Published:2018-12-27
  • Contact: WANG Shengchun, E-mail：wangsc@fmmu.edu.cn

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摘要： 目的：比较液体培养法、聚合酶链式反应（PCR）和实时荧光核酸恒温扩增检测技术（SAT）在解脲支原体(Uu)检测中的诊断价值。方法：应用液体培养法、PCR法和SAT法对77例疑似男性泌尿生殖系统感染患者尿道和23例女性宫颈的分泌物进行解脲支原体的检测。结果：100例患者中阳性29例（29.00%），其中男15例，感染率19.48%（15/77），女14例，感染率60.87%（14/23）。SAT法的敏感性为100%高于液体培养法89.66%和PCR法86.21%，差异无统计学意义(P>0.05)，SAT法的特异性为84.50%低于液体培养法的98.59%和PCR法的98.59%，差异有统计学意义(P<0.05)。结论：以上三种方法均可用来检测Uu，SAT敏感性强，特异性低，适用于检测病原微生物含量不高的标本。

关键词: 液体培养法, 聚合酶链式反应, 实时荧光核酸恒温扩增检测技术, 解脲支原体

Abstract: Objective: To compare the application of liquid culture, PCR and SAT in the diagnosis of Ureaplasma urealyticum (Uu). Methods: Uu was detected from specimens?of 77 males’urethra and 23 females’cervix by liquid culture, PCR and SAT. Results: The Uu was detected in 100 specimens, giving a positivity rate of 29%. Out of the 29 positive specimens, 15 (19.48%) were from males and 14 (60.87%) were from females. The sensitivity of SAT was 100% which was higher than liquid culture (89.66%) and PCR (86.21%). However, there were no significant differences among the three methods (P>0.05). The specificity of SAT was 84.50% which was lower than liquid culture (98.59%) and PCR (98.59%), with significant differences (P<0.05). Conclusion: Liquid culture, PCR and SAT can be used in the detection of Uu. SAT has higher?sensitivity and lower specificity, which is more suitable?for detecting?low?concentration of Uu.

Key words: liquid culture, PCR, SAT, ureaplasma urealyticum