中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (10): 580-584.

• 论著 • 上一篇    下一篇

双氢青蒿素对UVB诱导的人皮肤HaCaT细胞凋亡的保护作用

惠海英1  白静静1  孙晶莹1  刘杨1  肖生祥2   

  1. 1陕西省人民医院，陕西西安，710068
    2西安交通大学第二医院，陕西西安，710004
  • 出版日期:2017-10-15 发布日期:2018-12-06
  • 通讯作者: 惠海英，E-mail: haiyinghui@163.com

Protective effect of dihydroartemisinin on HaCaT cell apoptosis induced by UVB

HUI Haiying1, BAI Jingjing1, SUN Jingying1, LIU Yang1, XIAO Shengxiang2.   

  1. 1. Shaanxi Provincial People's Hospital, Xi'an 710068, China; 2. The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China
  • Online:2017-10-15 Published:2018-12-06
  • Contact: HUI Haiying, E-mail: haiyinghui@163.com

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摘要： 目的：明确双氢青蒿素(DHA)对UVB诱导人皮肤HaCaT细胞凋亡的保护作用。方法：将体外培养的HaCaT细胞分为空白对照组，UVB 照射组和UVB+DHA（20、40、60、80 μmol/L）组，采用 MTT法检测细胞系的增殖情况，流式细胞仪检测细胞的凋亡率，RT-PCR法检测抗凋亡基因survivin和促凋亡基因caspease-3(激活型)mRNA水平，Western检测相关蛋白表达水平。结果：30 mJ/cm2 UVB照射24 h后，UVB组和UVB+DHA（20、40、60、80 μmol/L）组细胞的增殖率分别为空白对照组的(50.13±2.42)%，(60.23±2.30)%,(69.24±3.19)%,(79.37±2.47)%和(70.41±2.24)%。UVB组和UVB+60 μmol/L DHA组中HaCaT细胞凋亡率分别为(46.7±3.2)%和(23.71±1.2)%。与UVB组比较，UVB+60 μmol/L DHA组中HaCaT细胞caspase-3 mRNA及蛋白表达降低、survivin mRNA及蛋白表达升高。结论：DHA可抑制UVB所致HaCaT细胞的凋亡，其机制可能与survivin和caspase-3有关。

关键词: 双氢青蒿素；HaCaT细胞；caspease-3, UVB

Abstract: Objective: To determine the protective effect of dihydroartemisinin (DHA) on HaCaT cells apoptosis induced by UVB. Methods: HaCaT cells cultured in vitro were divided into blank control group, UVB group and UVB+DHA (20, 40, 60, 80 μmol/L) group. The proliferation of HaCaT cells, cell apoptosis, mRNA levels of anti-apoptotic gene survivin and pro-apoptotic gene caspease-3 and corresponding protein levels were detected by MTT, flow cytometric analysis, qRT-PCR and Western blotting. Results: After the HaCaT cells was irradiated for 24 hs, the proliferation of the cells in the UVB group and UVB+DHA (20, 40, 60, 80 μmol/L) groups was (50.13±2.42)%, (60.23±2.30)%, (69.24±3.19)%, (79.37±2.47)% and (70.41±2.24)% of the blank control group. The apoptosis rate in the UVB group and UVB+60 μmol/L DHA group was (46.7±3.2)% and (23.71±1.2)%. Compared with the UVB group, the mRNA and protein levels of pro-apoptotic gene caspease-3 in the UVB+60 μmol/L DHA group was decreased and the levels of anti-apoptotic gene survivin was increased. Conclusion: DHA inhibits the apoptosis of HaCaT cells induced by UVB, which may be related with caspase-3 and survivin.

Key words: dihydroartemisinin, HaCaT cells, caspase-3, UVB