中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (7): 413-415.

• 论著 • 上一篇    下一篇

miR-203及其下游靶基因p63和survivin在瘢痕疙瘩皮损中的表达

庄乐  马伟元   

  1. 山东大学齐鲁医院皮肤科，济南，250012
  • 出版日期:2017-07-15 发布日期:2018-12-01
  • 通讯作者: 马伟元，E-mail：dermatologyqlh@163.com

Expression of miR-203 and its downstream targets p63 and survivin in the keloid lesions

ZHUANG Le, MA Weiyuan.   

  1. Department of dermatology, Qilu Hospital of Shandong University, Jinan 250012,China.
  • Online:2017-07-15 Published:2018-12-01
  • Contact: MA Weiyuan, E-mail：dermatologyqlh@163.com

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摘要： 目的: 检测瘢痕疙瘩皮损中miR-203及其下游靶基因p63和survivin mRNA的表达。方法: real-time PCR法检测30例瘢痕疙瘩皮损及30例健康对照皮肤中miR-203、p63和survivin  mRNA的表达。结果: 与健康对照皮肤相比，瘢痕疙瘩皮损中miR-203表达明显下调，其表达量为对照组的0.32±0.16倍（P＜0.05），靶基因p63和survivin mRNA表达明显上调，其表达量分别为对照组的3.90±0.84倍和5.11±0.93倍（均P＜0.05）。经Pearson相关性分析，miR-203与survivin间呈负相关（r=-0.40，P<0.05)，miR-203与p63间呈负相关（r=-0.51,P<0.05)。结论: miR-203及其下游靶基因p63和survivin可能参与了瘢痕疙瘩的发病过程。

关键词: miR-203, p63, survivin, 瘢痕疙瘩

Abstract: Objective: To detect the RNA expression levels of miR-203 and its downstream targets p63 and survivin in the lesions of keloid. Methods: The RNA levels of miR-203 and its downstream targets p63 and survivin from 30 lesions and 30 normal controls were detected by the quantitative real-time PCR technique. Results: The expression level of miR-203 in the lesions of keloid was dramatically down-regulated which was 0.32±0.16 of the normal controls (P＜0.05). While the expression levels of p63 and survivin in the lesions of keloid were sharply up-regulated which were 3.90±0.84 times and 5.11±0.93 times than those in the normal controls respectively（P＜0.05）. Conclusion: miR-203 and its downstream targets p63 and survivin are probably involved in the pathogenesis of keloid.

Key words: miR-203, P63, survivin, keloid