中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (1): 25-28.

• 论著 • 上一篇    下一篇

UVB对成纤维细胞中IFI16表达的影响

马慧敏1刘玮2   

  1. 1、首都医科大学电力教学医院 皮肤科 北京 100073 2、空军总医院 皮肤病医院 刘玮 北京 100042
  • 出版日期:2018-01-15 发布日期:2018-12-05

Up-regulation of IFI16 in fibroblasts irradiated by UVB

MA Huimin1 , LIU Wei2.   

  1. 1 Department of Dermatology, Electric Teaching Hospital of Capital Medical University，Beijing 100137, P.R. China; 2 Department of Dermatology, the General Hospital of the Air Force, Beijing 1000142, P.R. China
  • Online:2018-01-15 Published:2018-12-05

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摘要： 目的：检测UVB辐射后成纤维细胞中IFI16的表达水平。方法：用亚毒性UVB诱导成纤维细胞老化，并用real-time PCR和western bolt方法检测IFI16 mRNA 和蛋白质表达水平的变化。结果：UVB照射后诱导的老化成纤维细胞中IFI16 mRNA和蛋白质分别为空白对照组的2.11±0.45倍和1.59±0.23倍。结论： UVB诱导的成纤维细胞中IFI16表达水平升高，IFI16可能参与细胞光老化的进程。

关键词: IFI16, 细胞光老化

Abstract: Objective: To detect the level of IFI16 in the fibroblasts irradiated by UVB. Methods: The fibroblasts were irradiated by triplicate UVB, and the levels of IFI16 mRNA and protein were detected by RT-PCR and western blot. Results: The levels of IFI16 mRNA and protein in the fibroblasts irradiated by UVB were 2.11±0.45 and 1.59±0.23 times more than those in normal fibroblasts (P<0.05). Conclusion: The level of IFI 16 was increased in the fibroblasts irradiated by UVB. IFI 16 may be involved in the process of fibroblasts photoageing.