中国麻风皮肤病杂志 ›› 2019, Vol. 35 ›› Issue (1): 20-24.doi: 10.12144/zgmfskin201901020

• 论著 • 上一篇    下一篇

疏血通注射液对兔耳增生性瘢痕MMP-2和ɑ-SMA表达的影响

施建飞1  陈晓栋2   

  1. 1武警江苏省消防总队南通市消防支队卫生队，江苏南通，226001
    2南通大学附属医院皮肤科，江苏南通，226001
  • 出版日期:2019-01-15 发布日期:2019-02-26
  • 通讯作者: 陈晓栋，E-mail: dermatochen@163.com

Effect of Shuxuetong injection on the expression of MMP-2 and ɑ-SMA in hypertrophic scars of rabbit ear

SHI Jianfei1, CHEN Xiaodong2   

  1. 1. Armed Police Jiangsu Provincial Fire Brigade Nantong Fire Brigade Medical Team, Nantong 226001, China;  2. Department of Dermatology, Hospital Affiliated to Nantong University, Nantong 226001, China
  • Online:2019-01-15 Published:2019-02-26
  • Contact: CHEN Xiaodong, E-mail: dermatochen@163.com

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摘要： 目的：观察疏血通注射液对兔耳增生性瘢痕基质金属蛋白酶2（MMP-2）和ɑ-平滑肌肌动蛋白(ɑ-SMA)的影响。方法：选择新西兰白兔9只，创建兔耳增生性瘢痕动物模型，造模成功后30天（待创面上皮化后）,将其随机分为3组：疏血通组,曲安奈德组和空白对照组,每组3只。分组注射药物20天后取材，检测瘢痕厚度并计算瘢痕增生指数，苏木素-伊红（HE）和VG染色观察组织学结构和胶原纤维的表达，qRT- PCR 和免疫印迹法检测瘢痕组织中MMP-2和ɑ-SMA mRNA和蛋白的表达。结果：疏血通组瘢痕厚度和瘢痕增生指数,MMP-2 mRNA和蛋白水平，ɑ-SMAmRNA和蛋白水平分别为36.67±1.74和1.19±0.07，0.28±0.04， 0.53±0.06，1.40±0.34，0.56±0.06，曲安奈德组分别为35.33±3.51和1.25±0.15，0.18±0.01，1.03±0.17，0.50±0.03，0.39±0.03；空白对照组分别为85.00±7.38，2.77±0.37，1.01±0.07，0.83±0.06，4.43±0.47，0.81±0.05。HE和VG染色显示，疏血通组与曲安奈德组成纤维细胞及胶原纤维排列较空白对照组减少和稀疏。结论：疏血通注射液能够抑制瘢痕增生，降低MMP-2和ɑ-SMA蛋白的表达。

关键词: 增生性瘢痕, 疏血通, 曲安奈德, MMP-2, ɑ-SMA

Abstract: Objective: To determine the effect of Shuxuetong injection on the expression s of matrix metalloproteinase 2 (MMP-2) and ɑ-smooth muscle actin (ɑ-SMA) in hypertrophic scar of rabbit ear. Methods: Nine New Zealand white rabbits were selected randomly. Firstly we established a hyperplastic scar model of rabbit ears. When the wounds were reepithelialized after 30 days, the nine rabbits were randomly divided into three groups: blank control group, triamcinolone acetonide injection group and Shuxuetong injection group (3 rabbits in each group). After 20 days，the thickness of scar was measured and the scar hyperplasia index was calculated. The histological structure and the collagen fiber expression were observed by hematoxylin-eosin (HE) and VG staining. The levels of MMP-2 or α-SMA mRNA and protein levels were detected by qRT-PCR and Western Blot. Results: The scar thickness, scar hyperplasia index, the mRNA and protein level of MMP-2 and ɑ-SMA were 36.67±1.74, 1.19±0.07, 0.28±0.04, 0.53±0.06,1.40±0.34 and 0.56±0.06, respectively, in the Shuxuetong group, were 35.33±3.51, 1.25±0.15, 0.18±0.01, 0.39±0.03, 1.03±0.1 and 0.50±0.03, in the triamcinolone acetonide group, and were 85.00±7.38, 2.77±0.37, 1.01±0.07, 0.83±0.06, 4.43±0.47 and 0.81±0.05 in the blank control group. HE staining and VG staining showed that the fibroblasts and collagen fibers in the triamcinolone acetonide group and Shuxuetong group were less than that in the blank control.group. Conclusion: Shuxuetong injection can inhibit the proliferation of scar tissue by decreasing the expression of MMP-2 and α-SMA.

Key words: hypertrophic scar, Shuxuetong, triamcinolone acetonide, MMP-2, ɑ-SMA