Abstract: Objective: To prepare the epigallocatechin-3-gallate-psoralen liposome (EGCG-PL) and to detect the transdermal diffusion characteristics in vitro. Methods: EGCG-PL was prepared by reverse phase evaporation approach. The particle size, zeta potential and encapsulation efficiency of EGCG-PL were measured by laser scattering particle size distribution analyzer and ultrafiltration-HPLC method. The percutaneous diffusion characteristics of the EGCG-PL and EGCG-PS in vitro was compared by Franz diffuser device. Results: The EGCG-PL had an average size of (118.1±17.0) nm with surface charge of -29.3 mV. The encapsulation efficiency of EGCG, psoralen and isopsoralen in EGCG-PL was (85.58±1.07)%, (55.49±2.82)% and (53.73±1.29)%, respectively. The transdermal penetration study showed that the cumulative penetration amounts and drug retention amounts of the EGCG-PL were significant higher than that in EGCG-PS. Conclusion: The preparation technology of EGCG-PL is simple and the quality is stable.

Key words: epigallocatechin-3-gallate, psoralen, liposome, percutaneous absorption