China Journal of Leprosy and Skin Diseases ›› 2020, Vol. 36 ›› Issue (8): 467-472.doi: 10.12144/zgmfskin202008467

• Original Articles • Previous Articles     Next Articles

Expression of long non-coding RNA in Kaposi's sarcoma

LIU Zhiquan1, YUAN Hu2, PU Xiongming3   

  1. 1 Medical College of Shihezi University, Shihezi 832002, China; 
    2 Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine, Changsha 410006,China; 
    3 People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
  • Online:2020-08-15 Published:2020-08-11
  • Contact: PU Xiongming, E-mail: puxiongming@126.com

Abstract: Objective: To detect the differential expression of long non-coding RNA (lncRNA) in Kaposi's sarcoma (KS) tissues and normal adjacent tissues. Methods: The lncRNA expression profiles were detected by high-throughput lncRNA microarray technology in Kaposi's lesions and the adjacent normal tissues in 5 patients. The lncRNAs of differential expression between lesions and adjacent normal tissues were screened, and the results of the microarray were further verified by qRT-PCR. The KEGG pathway annotation of different lncRNA target genes was analyzed by bioinformatics. Results: A total of 717 lncRNAs of differential expression were screened, of which 408 were up-regulated and 309 were down-regulated in Kaposi's sarcoma tissues. The expression of lnc-PXDN-3:3 and lnc-PERP-10:2 in Kaposi's sarcoma tissues was all significantly higher, which was consistent with the chip results. Biological process annotation focuses on Wnt signal pathway, ubiquitin metabolism, TGF-beta signal pathway, p53 signal pathway and so on.Conclusion: lncRNA may be related to Kaposi's sarcoma.

Key words: Kaposi's sarcoma, long non-coding RNA, gene chip, qRT-PCR