China Journal of Leprosy and Skin Diseases ›› 2020, Vol. 36 ›› Issue (9): 533-536.doi: 10.12144/zgmfskin202009533

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Effect of Z-VAD-FMK, a broad caspase inhibitor, on the expression of cytokines released by CD4+T cell subset in the patients with atopic dermatitis

LI Yuanyuan1, HAO Baozhen2, MENG Yunfang1   

  1. 1 Department of Dermatology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China; 2 Shandong Provincial Maternity&Child-care Hospital, Jinan 250014, China
  • Online:2020-09-15 Published:2020-08-21
  • Contact: MENG Yunfang, E-mail: mengyunfang6.12@163.com

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Abstract: Objective: To determine the effect of Z-VAD-FMK, a broad caspase inhibitor, on the expression of cytokines released by CD4+T cells subset in the patients with atopic dermatitis (AD). Methods: PBMCs from 30 AD patients were co-cultured with Z-VAD-FMK solution in vitro, then the cells were divided into PBS group, Z-VAD-FMK group and dexamethasone group. The levels of CD4+T cells were detected by flow cytometry. The level of IFN-γ, IL-4 and IL-17 were detected by ELISA. Results: The levels of IFN-γ, IL-4 and IL-17 in the PBS group were higher than that in the Z-VAD-FMK group (Ps<0.01) and there was no significant differences of these between the Z-VAD-FMK group and dexamethasone group (Ps>0.05). Conclusion: Z-VAD-FMK can inhibit the expression of cytokines released by CD4+T cells subset of Th1, Th2 and Th17 in patients with AD.

Key words: dermatitis, atopic, caspases, CD4+T cells