Abstract: Objective: To investigate the susceptibility of Neisseria gonorrhoeae to ceftriaxone and cefixime in Zhongshan, and the genotypes of N. gonorrhoeae multi-antigen sequencing typing (NG-MAST). Methods: Neisseria gonorrhoeae strains were collected from medical institutions in Zhongshan area from 2017 to 2018. After separation, identification and purification, the minimum inhibitory concentrations (MICs) of ceftriaxone and cefixime in N. gonorrhoeae were confirmed by the agar dilution method. The genotyping of the porB and tbpB was performed by NG-MAST. Results: A total of 193 strains of Neisseria gonorrhoeae were detected, of which 5.2% and 13.5% showed low susceptibility to ceftriaxone and cefixime, respectively. NG-MAST showed that there were 125 types in 193 strains, among which 65 were known types and 60 were new ones. The most prevalent NG-MAST sequence types (STs) were ST5308, ST5061, ST2268, ST10091, NEW1, NEW2 and NEW3, and the MIC values of the same type to the same antibiotic were the same or similar. Conclusion: The low susceptibility of N. gonorrhoeae isolates to ceftriaxone and cefixime has already existed. The new genotypes of the outer membrane protein of Neisseria gonorrhoeae porB and tbpB appear. 

Key words: Neisseria gonorrhea, ceftriaxone, cefixime, NG-MAST