Abstract: Objective: To compare the performance differences of three methods (ECLIA,CLIA, ELISA) in the detection of Treponema pallidum specific antibody. Methods: 60 cases of positive samples and 60 cases of negative samples were screened by TPPA test, all samples were tested for Treponema pallidum specific antibody by ECLIA, CLIA and ELISA. By using receiver operating curve (ROC) to define the value of different methods in the diagnosis of syphilis, determine the best truncation value of S/CO, and compare AUC size of different methods. Results: When S/CO ≥5 of ECLIA, S/CO≥7 of CLIA or S/CO≥7 of ELISA, TPPA were ture positive. The AUC, sensitivity, specificity, and coincidence rate of ECLIA and CLIA were higher than those of ELISA, with significant differences (Ps<0.05). Conclusion: When the S/CO of ECLIA, CLIA and ELISA is less than 5, 7 and 7 respectively, TPPA should be used for verification to reduce misdiagnosis. The diagnostic value of ECLIA and CLIA is higher than ELISA. 

Key words: treponema pallidum specific antibody, ECLIA, CLIA, ELISA