Abstract: Objective: To evaluate the application of immunofluorescent staining method in detecting the fungi in skin tissues. Methods: A total of 43 formalin-fixed paraffin-embedded tissues which were indentified as fungal infection by pathology and fungal culture were stained with Calcofluor White and Periodic acid-Schiff （PAS）stain. Results: In 43 cases, 27 cases (62.8%) were positive of PAS, 30 cases (69.7%) were positive of immunofluorescent staining, with no significant difference (P>0.05). There were 5 patients both negitive of immunofluorescent staining and PAS and the positivity rate was 88.4% of two methods combined.  Conclusion: Fluorescent staining is an supplement for detection of fungi in skin tissues.

Key words: immunofluorescent staining, dermatomycosis, biospy