China Journal of Leprosy and Skin Diseases ›› 2022, Vol. 38 ›› Issue (10): 673-677.doi: 10.12144/zgmfskin202210673

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Ebastine induces autophagy in human melanoma cells by inhibiting AKT/mTOR pathway

ZHANG Ping1，2, WANG Jing3, WANG Yingchao1，4, NI Li4, LI Mingming4, DANG Ningning4   

  1. 1 Cheeloo College of Medicine, Shandong University, Jinan 250012, China; 2 Jinan Central Hospital, Shandong University, Jinan 250013, China; 3 Department of Dermatology, Binzhou Medical University Hospital, Binzhou 256600, China; 4 Shandong Provincial Hospital, Shandong First Medical University, Jinan 250021, China
  • Online:2022-10-15 Published:2022-08-22

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Abstract: Objective: To study the effect and mechanism of ebastine on autophagy of human melanoma cells. Methods: The human melanoma cells A375 and M14 were cultured in vitro and cell viability was detected by a cell counting kit (CCK-8) and IC50 was calculated. The autophagy flow was detected by mCherry-EGFP-LC3B fluorescence plasmid. Western blot was used to detect the expression level of autophagy-related proteins LC3, Beclin1 and signaling pathway proteins. Results: After the action of ebastine, the cell viabilities of human melanoma cells decreased, the production of autophagosomes and autophagolysosomes in human melanoma cells increased, the ratio of LC3-Ⅱ/Ⅰ and the expression of Beclin1 increased, the activation of AKT/mTOR signaling pathway decreased, and p-AKT/AKT and p-mTOR/mTOR in human melanoma cells decreased. Conclusion: Ebastine induces significant autophagy in human melanoma cells by inhibiting the AKT/mTOR signaling pathway.

Key words: ebastine, human melanoma cells, autophagy, AKT/mTOR signaling pathway