Abstract: Object To determine the effect of myricetin on cell proliferation and collagen synthesis of human fibroblast. Methods: The primary cultured fibroblasts in vitro were isolated and treated with myricetin in different concentration (0, 10, 25, 50, 75, 100μM). Cell cycle was measured by flow cytometry. The fibroblasts proliferation and the expression of collagen type I was assessed by MTT assay. Results: The cellular viability of fibroblast was increased in the dose of 10 μM and 25 μM of myricetin, and was decreased in the dose of 50μM or above. Conclusions: The proliferation and collagen synthesis of human fibroblast can be inhibited when the concentration of myricetin is higher than 50μM, which provides a theoretical base for the treatment of fibrosis diseases.

Key words: fibroblast；myricetin, collagen