China Journal of Leprosy and Skin Diseases ›› 2017, Vol. 33 ›› Issue (12): 705-708.

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Up-regulation of RGS4 inhibits the proliferation and migration of human malignant melanoma cells in vitro

XUE  Xiaotong1,2, JIAO jing3, ZHANG qianqian3, HUANG Shuhong1, DANG Ningning2.   

  1. 1. School of Medicine, Shandong University, Jinan 250012,China；
    2. Department of Dermatology, Jinan Central Hospital affiliated to Shandong University, Jinan 250013,China；
    3. Department of Dermatology,The Chinese People's Liberation Army 88 Hospital, Taian 271000, China
  • Online:2017-12-15 Published:2018-12-12
  • Contact: DANG Ningning，E-mail: 15318816250@163.com

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Abstract: Objective: To determine the influence of pcDNA3.1-RGS4 vector up-regulating RGS4 expression on the proliferation and migration of human malignant melanoma cells in vitro. Methods: The cells were cultured in vitro, then divided into the control group (pcDNA3.1) and experimental group (pcDNA3.1- RGS4). The expression of RGS4 mRNA and protein in M14 cell was detected by RT-PCR and Western blot. The proliferation of M14 cell was detected by CCK-8 and colon formation assay. The migration of M14 cell was detected by wound healing assay. Results: The levels of RGS4 mRNA and protein in the experimental group were 3.25×103 and 6.73 times more than that in the control group (Ps<0.05). The OD scores and clone number of M14 cells in the experimental group were 1.33±0.07 and 34±5.53, which was lower than that in the control group (1.61±0.11 and 56±7.68) (Ps<0.05). M14 cellular migration ability in the experimental group was weaker than that in the control group. Conclusion: RGS4 up-regulation inhibits the M14 cell proliferation and migration in malignant melanoma. RGS4 may play an important role in the development of malignant melanoma.

Key words: GPCR, RGS4 gene, malignant melanoma, proliferation and migration