清热利湿饮,Cyclin E,泛素化,FBW7 ," /> 清热利湿饮,Cyclin E,泛素化,FBW7 ,"/> Qingrelishi recipe,Cyclin E,ubiquitination,FBW7 ,"/> <p class="MsoNormal"> The expression of Cyclin E affected by Qingrelishi recipe through regulating FBW7 in HaCaT cells

China Journal of Leprosy and Skin Diseases ›› 2017, Vol. 33 ›› Issue (3): 149-152.

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The expression of Cyclin E affected by Qingrelishi recipe through regulating FBW7 in HaCaT cells

SUN Shuna1, HUANG Qiteng1, WEI Haifeng2, LI Guangyuan3, ZHAO Ying1, ZHANG Xiaojie1   

  1. 1. Department of Dermatology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250012, China; 2. Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 3. Agricultural Bank of China Shandong Branch, Jinan 250014, China

  • Online:2017-03-15 Published:2018-12-14
  • Contact: ZHANG Xiaojie, E-mail: qlzxj@126.com

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Abstract:

Objective: To determine the mechanism of the expression of Cyclin E  affected by Qingrelishi recipe in HaCaT cells. Methods: Half-life of Cyclin E in HaCaT cells was detected by Western blot. The ubiquitination level of Cyclin E was detected through IP and Western blot. The level of ubiquitin ligase E3 SCF-FBW7 affected by Qingrelishi recipe was detected by Western blot and Real-time PCR. Results: Compared with control group, the Half-life of Cyclin E was shorter, the ubiquitination level and the degradation speed of Cyclin E were higher and the level of FBW7 mRNA and protein was higher in the Qingrelishi recipe group. Conclusion: Qingrelishi recipe can inhibit the Cyclin E expression through the regulation of FBW7

Key words: Qingrelishi recipe')">

Qingrelishi recipe, Cyclin E, ubiquitination, FBW7