Abstract: Objectives: To establish the mice model of dexamethasone osteoporosis and to determine the effect of Xianzhengukang capsule on the therapeutic effect index. Methods: Forty C57BL / 6 mice were divided into dexamethasone group (group A), Xianzhengukang group (group B), dexamethasone + Xianzhengukang group (group C), blank control group (group D) randomly. The mice in the group A, B, C group were respectively treated with gluteal muscle injection of dexamethasone sodium phosphate twice a week for 6 weeks, Xianzhengukang mixed suspension 20 mL/kg ,once a day, dexamethasone sodium phosphate+ Xianzhengukang. Bone mineral density and osteoblast shape were detected by multi-mode living imaging system and transmission electron microscopy (Tem).The level of blood calcium, phosphorus, parathyroid hormone (PTH), type I collagen before in mice serum C - peptide (PICP), osteocalcin (BGP), bone alkaline phosphatase (BALP), estradiol (E2), calcitonin (CT) and, progesterone (PROG) were detected. Results: The level of bone mineral density in the group A was lower than that in the group D (P<0.05) and there was no significant differences between the group C and group D (P>0.05). The osteoblast in the group A was dissolved, that in the group B was normal. The osteoblast?nuclei were ovoid and secreted collagen in the group C and D. The level of PTH in the group A, B, C were 46.03±6.390017, 88.46±9.361211, 67.17±5.236196 and 59.71±5.552481, with a significant difference (P<0.05); that of PICP were 5.59±0.839515, 10.52±1.046153, 7.99±0.711197 and 7.38±0.72189, with a significant difference (P<0.05); that of BGP were 8.59±0.813074, 4.27±0.809171, 7.25±0.847148 and 7.77±0.753058, with a significant difference (P<0.05); that of BALP were 2.50±0.40932, 4.79±0.528068, 3.56±0.314464 and 3.34±0.368841, with a significant difference (P<0.05); that of E2 were 47.35±4.455982, 26.05±3.840661, 38.38±3.293764 and 36.42±3.326303, with a significant difference (P<0.05); that of PROG were 8.87±0.872026, 4.37±0.79088, 6.34±0.706422 and 6.38±0.743752, with a significant difference (P<0.05); that of CT were 27.93±3.315907, 15.97±2.406497, 19.61±1.820368,23.06±1.973241, with a significant difference (P<0.05). Conclusions: Xianzhengukang capsule promote bone formation and increased bone mineral density, which may through up-regulating the level of PTH、PICP、BALP and down-regulating the level of BGP、E2、CT and PROG.

Key words: bone?mineral?density, glucocorticoid osteoporosis;Xianzhengukang capsule