China Journal of Leprosy and Skin Diseases ›› 2018, Vol. 34 ›› Issue (6): 340-344.

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Itraconazole inhibits the migration of murine bone marrow derived dendritic cells and secretion of MMP-2, MMP-3, MMP-12 and RANTES

ZHENG Xiaoli1, LIANG Guanzhao2, SHI Dongmei3, SHEN Yongnian2, LIU Weida2, CHEN Guanzhi1   

  1. 1.Department of Dermatology, the Affiliated Hospital of Qingdao University, Qingdao 266003, China; 2.Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China; 3. Department of Dermatology, Jining No. 1 People’s Hospital, Jining 272011, China
  • Online:2018-06-15 Published:2018-12-11
  • Contact: CHEN Guanzhi, E-mail：chenguanzhiqd@126.com

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Abstract: Objective: To determine the effects of itraconazole on the migration of murine bone marrow derived dendritic cells (DCs) and the secretion of MMPs and RANTES. Methods: DCs were cultivated for 8 days with rmGM-CSF. DCs were divided into the control group and itraconazole (0.25, 0.5, 1 μM ) group. The viability and migration of DCs were determined by CCK-8 assay and transwell assay respectively. The levels of MMP-2, MMP-3, MMP-8, MMP-12 and RANTES were detected by Luminex. The levels of MHCII, CD80, CD86 and CCR7 were measured by flow cytometry. Results: The cytotoxicity of itraconazole on DCs was time- and dose-dependent. The migration rate and the levels of MMP-2, MMP-3, MMP-12 and RANTES in the itraconazole group were lower than those in the control group (Ps<0.05). There was no significant difference in the level of CCR and MMP-8 between the two groups (P>0.05). Conclusion: Itraconazole can inhibit the DCs cell migration and secretion of MMPs and RANTES.

Key words: itraconazole, DCs, migration, MMPs, RANTES