Abstract: Objective: To prepare tea polyphenol binary ethosomes (TP-EL) and evaluate its quality. Methods: Encapsulation efficiency was taken as evaluation index to optimize the formula and preparation technology of TP-EL. The quality of TP-EL was assessed. Results: The optimal formula and preparation technology were as follows: the concentration of phosppholipids was 4%, the ratio of phospholipids /cholesterol was 5:1, the ratio of TP/phospholipids was 1:8 and the stirring time was 40 min. The results showed that most of the TP-EL was unilamellar structure. The TP-EL had an average size of 123.0 ± 13.2 nm with polymer dispersity index (PDI) of 0.15 ± 0.07. The surface charge of TP-EL was -35.2 mV. Moreover, TP was readily incorporated into ethosomes with encapsulation efficiency of 71.82±2.03%. Conclusion: The preparation technology for TP-EL was simple and stable.

Key words: tea-polyphenol, binary ethosomes, encapsulation efficiency, stability