Abstract: Objective: To determine the effects of NB-UVB on the expression of CCL22 from HaCaT cells. Methods: The cultrued HaCaT cells were divided into two groups. The HaCaT cells in one group were treated with different dose of NB-UVB (0、100、200、400 mJ/cm2) and those in the second group were treated with NB-UVB (400 mJ/cm2) after the pretreatment with PDTC. The expression of CCL22 was detected by Real-time PCR and ELISA. The level of NF-κB p65 phosphorylation was detected by western blot. Results: The expression of CCL22 and the level of NF-κB p65 phosphorylation in HaCaT cells treated with NB-UVB were increased as the dose of of NB-UVB was increasing. The expression of CCL22 and the level of NF-κB p65 phosphorylation in HaCaT cells pretreated by PDTC were lower than those treated with NB-UVB only and the higher the concentration of PDTC, the effects were more obvious. Conclusion: The CCL22 expression induced by NB-UVB in keratinocytes may through activation of NF-κB pathway.

Key words: NB-UVB, keratinocytes, CCL22