中国麻风皮肤病杂志 ›› 2019, Vol. 35 ›› Issue (6): 342-345.doi: 10.12144/zgmfskin201906342

• 论著 • 上一篇    下一篇

表没食子儿茶素没食子酸酯-补骨脂脂质体的制备及体外透皮性质研究

宋娟  吕成志  王栋  丁峰  于绪东   

  1. 大连市皮肤病医院药剂科，辽宁大连，116021
  • 出版日期:2019-06-15 发布日期:2019-06-06
  • 通讯作者: 王栋，E-mail：dlahdong@163.com

Preparation of epigallocatechin-3-gallate-psoralen liposome and detection of transdermal penetration in vitro

SONG Juan, LYU Chengzhi, WANG Dong, DING Feng, YU Xudong   

  1. Department of Pharmacy, Dalian Dermatosis Hospital, Dalian 116021,China
  • Online:2019-06-15 Published:2019-06-06
  • Contact: WANG Dong, E-mail：dlahdong@163.com

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摘要：

目的：制备表没食子儿茶素没食子酸酯-补骨脂脂质体（EGCG-PL）并检测其体外透皮性。方法：采用逆向蒸发法制备EGCG-PL，以激光粒度仪测定EGCG-PL的粒度及Zeta电位，超滤-HPLC法测定包封率，Franz 扩散装置比较EGCG-PL与EGCG-补骨脂醇溶液组（EGCG-PS）体外透皮性质的差异。结果：所制备的EGCG-PL粒径分布均匀，平均粒径为(118.1±17.0)nm，Zeta电位为-29.3 mV。EGCG-PL中EGCG、补骨脂素及异补骨脂素的包封率分别为(85.58±1.07)%、(55.49±2.82)%和(53.73±1.29)%。体外透皮试验结果显示，EGCG-PL中EGCG、补骨脂素及异补骨脂素24 h单位面积累积透过量及皮肤滞留量均明显高于EGCG-PS。结论：EGCG-PL制备简便、质量稳定。

关键词: 表没食子儿茶素没食子酸酯, 补骨脂, 脂质体, 透皮吸收

Abstract: Objective: To prepare the epigallocatechin-3-gallate-psoralen liposome (EGCG-PL) and to detect the transdermal diffusion characteristics in vitro. Methods: EGCG-PL was prepared by reverse phase evaporation approach. The particle size, zeta potential and encapsulation efficiency of EGCG-PL were measured by laser scattering particle size distribution analyzer and ultrafiltration-HPLC method. The percutaneous diffusion characteristics of the EGCG-PL and EGCG-PS in vitro was compared by Franz diffuser device. Results: The EGCG-PL had an average size of (118.1±17.0) nm with surface charge of -29.3 mV. The encapsulation efficiency of EGCG, psoralen and isopsoralen in EGCG-PL was (85.58±1.07)%, (55.49±2.82)% and (53.73±1.29)%, respectively. The transdermal penetration study showed that the cumulative penetration amounts and drug retention amounts of the EGCG-PL were significant higher than that in EGCG-PS. Conclusion: The preparation technology of EGCG-PL is simple and the quality is stable.

Key words: epigallocatechin-3-gallate, psoralen, liposome, percutaneous absorption