中国麻风皮肤病杂志 ›› 2019, Vol. 35 ›› Issue (9): 536-538.doi: 10.12144/zgmfskin201909536

• 论著 • 上一篇    下一篇

表没食子儿茶素没食子酸酯明胶纳米粒的制备及体外透皮性质研究

宋娟  于绪东  王栋   

  1. 大连市皮肤病医院药剂科，辽宁大连，116021
  • 出版日期:2019-09-15 发布日期:2019-09-16
  • 通讯作者: 宋娟，E-mail: sj271205219@163.com

Preparation of epigallocatechin-3-gallate-gelatin nanoparticles and detection of transdermal penetration in vitro

SONG Juan, YU Xudong, WANG Dong   

  1. Department of Pharmacy, Dalian Dermatosis Hospital, Dalian 116021, China
  • Online:2019-09-15 Published:2019-09-16
  • Contact: SONG Juan, E-mail: sj271205219@163.com

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摘要： 目的：制备表没食子儿茶素没食子酸酯-明胶纳米粒(EGCG-N)并研究其体外透皮性。方法：以激光粒度仪测定EGCG-N的粒度及Zeta电位，超滤-HPLC法测定EGCG-N的载药量及包封率，Franz扩散装置检测EGCG-N体外透皮性。结果：所制备的EGCG-N粒径分布均匀，平均粒径为(72.63±0.47)nm，Zeta电位为-28.0mV。EGCG-N中EGCG的载药量和包封率分别为(29.82±2.16)%和(83.91±0.93)%。EGCG-N中EGCG 24 h单位面积累积透过量及皮肤滞留量分别为(133.77±22.79)μg/cm2和(492.57±37.68)μg/g均明显高于EGCG溶液剂(53.30±21.12)μg/cm2和(165.88±17.96)μg/g(均P<0.01)。结论：EGCG-N制备简便，能够提高EGCG的经皮渗透能力。

关键词: 表没食子儿茶素没食子酸酯, 纳米粒, 透皮吸收

Abstract: Objective: To prepare the epigallocatechin-3-gallate-gelatin nanoparticles (EGCG-N) and investigate its in vitro transdermal diffusion characteristics. Methods: The particle size, zeta potential and encapsulation efficiency of EGCG-N were measured by laser scattering particle size distribution analyzer and ultrafiltration-HPLC method. The percutaneous diffusion characteristics of the EGCG-N were detected by Franz diffuser device. Results: The EGCG-N had an average size of (72.63±0.47) nm with surface charge of -28.0 mV. The drug-loading capacity and encapsulation efficiency of EGCG were (29.82±2.16)% and (83.91±0.93)%, respectively. The cumulative penetration concentration and drug retention concentration of the EGCG-N were (133.77±22.79)μg/cm2 and (492.57±37.68)μg/g, respectively,which were significant higher than that in EGCG-solution (53.30±21.12)μg/cm2 and (165.88±17.96)μg/g (Ps<0.01). Conclusion: EGCG-N is prepared simply and can improve the precutaneous permeability of EGCG.

Key words: epigallocatechin-3-gallate, nanoparticles, percutaneous absorption