中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (6): 339-342.doi: 10.12144/zgmfskin202006339

• 论著 • 上一篇    下一篇

低氧对恶性黑素瘤A375细胞中Nodal及细胞迁移相关蛋白表达的影响

谭自敏1,杨成莲2,钟桂书1,曾凡才3   

  1. 1西南医科大学附属医院皮肤科,四川泸州，646000;2绵阳市第三人民医院皮肤科，四川绵阳，621000；3西南医科大学生物化学与分子生物学实验室,四川泸州，646000
  • 出版日期:2020-06-15 发布日期:2020-06-04
  • 通讯作者: 钟桂书， E-mail：zhongguishu2009@163.com

Effect of hypoxia on the expression of Nodal and the impact of relative migration protein in malignant melanoma A375 cells

TAN Zimin1, YANG Chenglian2, ZHONG Guishu1, ZENG Fancai3   

  1. 1 Department of Dermatology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China; 2 Department of Dermatology, Mianyang Third People's Hospital, Mianyang 621000, China; 3 Department of Biochemistr, Southwest Medical University, Luzhou 646000, China
  • Online:2020-06-15 Published:2020-06-04
  • Contact: ZHONG Guishu, E-mail: zhongguishu2009@163.com

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摘要： 目的：明确低氧对恶性黑素瘤A375细胞中Nodal及细胞迁移相关蛋白表达的影响。方法：体外培养A375细胞，CoCl2构建细胞内低氧模型。细胞分为空白对照组，CoCl2组，SB-431542（Nodal抑制剂）组和CoCl2 +SB-431542组。通过Western Blot技术检测Nodal、类激活素激酶4/7（ALK4/7）、与恶性肿瘤进展相关的上皮钙黏附蛋白（E-cadherin）、基质金属蛋白酶-2（MMP 2)和波形蛋白(Vimentin)的表达水平。结果：CoCl2组细胞内Nodal及ALK7蛋白浓度，高于其他组（P<0.05）。各处理组均没有检测到ALK4蛋白；CoCl2组细胞中Vimentin和MMP2蛋白的浓度高于其他组（P<0.05）；各处理组E-cadherin蛋白水平差异不具统计学意义。结论：低氧环境可诱导A375细胞中Nodal和细胞迁移相关蛋白MMP2和Vimentin的高表达。

关键词: 恶性黑素瘤, Nodal, E-cadherin, MMP2, Vimentin

Abstract: Objective: To determine the effect of hypoxia on the expression of Nodal and the impact of relative migration protein in malignant melanoma A375 cell. Methods: Malignant melanoma A375 cells were cultured in vitro and treated with chloride (CoCl2) to construct an intracellular hypoxia model. The cells were divided into blank control group CoCl2 group, SB-431542 group and CoCl2 +SB-431542 group. The level of Nodal, Activin receptor-like kinase 4/7 (ALK4/7), E-cadherin, matrix metalloproteinase-2 (MMP-2) and vimentin was detected by Western blot. Results: The level of Nodal and ALK7 in A375 cells was higher in CoCl2 group than in other groups, with significant differences (Ps<0.05). ALK4 was not detected in all groups. The level of Vimentin and MMP2 in A375 cells was higher in CoCl2 group than in other groups, with significant differences (Ps<0.05). There was no significant difference of E-cadherin protein in different groups (P>0.05). Conclusion: Hypoxia can increase the expression of Nodal and relative migration protein of MMP2 and vimentin in the malignant melanoma A375 cells.

Key words: malignant melanoma, Nodal, E-cadherin, MMP2, Vimentin