中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (9): 515-518.doi: 10.12144/zgmfskin202009515

• 论著 • 上一篇    下一篇

N-乙酰半胱氨酸对PM2.5诱导肥大细胞活化作用的影响

林志鹏,李锦濯,曾倩雯,黄显琼,孙仁山   

  1. 陆军军医大学大坪医院皮肤科，重庆，400042
  • 出版日期:2020-09-15 发布日期:2020-08-21
  • 通讯作者: 孙仁山，E-mail: pharsunr@126.com

Effect of N-acetylcysteine on mastocyte activation induced by PM2.5

LIN Zhipeng, LI Jinzhuo, ZENG Qianwen, HUANG Xianqiong, SUN Renshan   

  1. Department of Dermatology, Daping Hospital, Army Medical University, Chongqing 400042, China
  • Online:2020-09-15 Published:2020-08-21
  • Contact: SUN Renshan, E-mail: pharsunr@126.com

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摘要： 目的：明确N-乙酰半胱氨酸（NAC）对颗粒物PM2.5诱导肥大细胞P815活化作用的影响。方法：用100 μg/mL PM2.5刺激肥大细胞建立脱颗粒模型，设空白组，模型组，不同浓度NAC组（浓度分为100，50，25 μmol/L）；不同浓度NAC处理肥大细胞6 h后，再以 PM2.5刺激6 h，CCK-8法测定细胞增殖活性，酶标仪测定活性氧（ROS）水平及β-氨基己糖苷酶（β-Hex）水平、ELISA检测IL-4水平，Western blot检测蛋白酶激活受体2（PAR2）蛋白表达水平。结果：与空白组比较，模型组ROS、β-Hex、IL-4含量及PAR2蛋白表达显著增高（均P＜0.05）；与模型组比较，NAC组肥大细胞ROS、β-Hex、IL-4含量及PAR2蛋白表达水平降低（均P＜0.05），其中50 μmol/L NAC组降低最明显。结论：NAC可降低肥大细胞氧化应激，抑制PAR2蛋白表达，减少炎症介质释放。

关键词: N-乙酰半胱氨酸, PM2.5, 肥大细胞, 蛋白酶激活受体2

Abstract:

Objective: To determine the effect of N-acetylcysteine (NAC) on mastocyte activation induced by PM2.5. Methods: The mastocyte degranulation model in vitro was established under inducing with 100 μg/mL PM2.5. Then the mastocytes were divided into the normal group, model group, NAC groups in different concentrations (100, 50, and 25 μmol/L). After cultured with different concentrations of NAC for 6 h, mastocytes were treated with PM2.5 for another 6 h. Cell proliferation activity was detected by CKK-8. The level of reactive oxygen species (ROS) production and β-hexosaminidase (β-Hex) was detected by microplate spectrophotometer. The level of IL-4 and protease-activated receptors-2 (PAR2) protein were measured by ELISA and western blot respectively. Results: Compared with the normal group, the levels of ROS production, β-Hex, IL-4 and PAR2 protein were significantly increased in the model group (Ps＜0.05). Compared to the model group, the levels of ROS production, β-Hex, IL-4 levels and PAR2 protein expression in the NAC groups were significantly lower (Ps＜0.05), and the decreased level was the most in 50 μmol/L NAC group. Conclusion: NAC can inhibit the oxidative stress, expression of PAR2 protein, and release of inflammatory mediators in vitro.

Key words: N-acetylcysteine, PM2.5, mastocytes, protease-activated receptors-2