中国麻风皮肤病杂志 ›› 2021, Vol. 37 ›› Issue (1): 26-29.doi: 10.12144/zgmfskin202101026

• 短篇论著 • 上一篇    下一篇

18β-甘草次酸新衍生物对HaCaT细胞增殖的影响

罗丽梅1,李创2,丁锐3,胥秀英3,郑一敏3   

  1. 1重庆化工职业学院制药工程学院，重庆，401120；
    2重庆大学环境与生态学院，重庆，400030；
    3 重庆理工大学，重庆，400054
  • 出版日期:2021-01-15 发布日期:2021-01-05
  • 通讯作者: 郑一敏，E-mail: zym19630507@163.com

Effect of 18β-glycyrrhetinic acid new derivatives on proliferation of HaCaT cells

LUO Limei1, LI Chuang2, DING Rui3, XU Xiuying3, ZHENG Yimin3   

  1. 1 College of Pharmaceutical Engineering，Chongqing Vocational College of Chemical Industry，Chongqing 401120, China; 
    2 College of Environment and Ecology, Chongqing University, Chongqing 400030, China; 
    3 College of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China
  • Online:2021-01-15 Published:2021-01-05
  • Contact: ZHENG Yimin, E-mail: zym19630507@163.com

PDF (PC)

212

摘要： 目的：明确18β-甘草次酸新衍生物3-O-(5-甲基吡嗪-2-甲酰)-甘草次酸（GA2）对角质形成细胞增殖的影响。方法：体外培养HaCaT细胞，分为DMSO空白对照组，不同浓度GA2(31.2、62.5、125 μM)组，CCK8、EdU法、细胞克隆、流式细胞术检查各组HaCaT细胞的增殖情况，Caspase 3活性检测法检测HaCaT细胞中Caspase 3表达水平。结果：不同浓度GA2作用HaCaT细胞24 h后，细胞抑制率和凋亡率随浓度的增加而升高；细胞克隆及细胞增殖随浓度的增加而减少；Caspase 3的表达水平随着GA2浓度的升高而增加。结论：GA2对HaCaT细胞有增殖抑制作用，该作用可能与激活Caspase 3有关。

关键词: GA2, HaCaT细胞, 增殖抑制

Abstract: Objective: To determine the effect of 18β-glycyrrhetic acid new derivative 3-O-(5-methylpyrazine-2-formyl)-glycyrrhetinic acid (GA2) on the proliferation of HaCaT cells. Methods: HaCaT cells were cultured in vitro, and then, the cells were divided into the blank control group, GA2 groups with different concentrations of (31.2, 62.5, 125 μM). The proliferation of HaCaT cells was detected by CCK8, EdU method, cell cloning, and flow cytometry. The Caspase 3 expression level was detected through Caspase 3 activity. Results: After HaCaT cells treated with 31.2, 62.5 and 125 μM of GA2 for 24 hours, the cell inhibition rates and apoptosis rates were increased in a dose-dependent manner. The cell cloning and cell proliferation ability were decreased in a dose-dependent manner. The expression level of Caspase 3 was increased in a dose-dependent manner. Conclusion: GA2 can inhibit the proliferation of HaCaT cells, which may be related to the activating Caspase 3.

Key words: GA2, HaCaT cells, inhibition