中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (11): 663-666.doi: 10.12144/zgmfskin202011663

• 论著 • 上一篇    下一篇

龙胆泻肝汤激活Bax/Caspase-3通路并诱导人角质形成细胞凋亡

杨冠群1,郭春1,魏海峰2,周煜1,周曙杰1,梁晨希1,吴国境1,许博涵1,邹永新1,张晓杰3,孙淑娜3   

  1. 1山东大学齐鲁医学院基础医学院遗传学系，山东济南，250012；

    2山东中医药大学，山东济南，250355；

    3山东中医药大学附属医院皮肤科，山东济南，250011

  • 出版日期:2020-11-15 发布日期:2020-10-30
  • 通讯作者: 孙淑娜，E-mail: 445178992@qq.com

Longdanxiegan decoction activates Bax/Caspase-3 pathway and induces the apoptosis of HaCaT cells

YANG Guanqun1, GUO Chun1, WEI Haifeng2, ZHOU Yu1, ZHOU Shujie1, LIANG Chenxi1, WU Guojing1, XU Bohan1, ZOU Yongxin1, ZHANG Xiaojie3, SUN Shuna3   

  1. 1 Department of Genetics, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China;
    2 Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 
    3 Department of Dermatology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China
  • Online:2020-11-15 Published:2020-10-30
  • Contact: SUN Shuna, E-mail: 445178992@qq.com

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摘要： 目的：明确龙胆泻肝汤对人角质形成细胞株HaCaT细胞凋亡的影响。方法：Tunel法检测龙胆泻肝汤对HaCaT细胞凋亡的影响; DAPI染色后荧光显微镜观察HaCaT细胞凋亡形态变化; Western blot检测分析HaCaT细胞凋亡相关蛋白表达。结果：龙胆泻肝汤处理组HaCaT细胞形态发生了明显变化，凋亡比例明显高于未处理细胞组。龙胆泻肝汤作用于HaCaT细胞后活化的caspase-3和Bax的表达明显上调， Bcl-2蛋白表达下降。caspase-3特异性抑制剂Z-DEVD-FMK预处理后可降低龙胆泻肝汤诱导的细胞凋亡。结论：龙胆泻肝汤能明显诱导人角质形成细胞的凋亡，其机制可能与下调Bcl-2的表达，增高Bax和caspase-3的表达有关。

关键词: 龙胆泻肝汤, HaCaT细胞, 银屑病, 细胞凋亡

Abstract: Objective: To determine the role of Longdanxiegan decoction in the apoptosis of HaCaT cells. Methods: The effect of Longdanxiegan decoction on the apoptosis of HaCaT cells was detected by Tunel method. The apoptosis of HaCaT cells was detected by fluorescence microscopy after DAPI staining. The effect of HaCaT cells on the expression of apoptosis-related proteins caspase-3, Bcl-2 and Bax was detected by Western blot. Results: The abnormal cells shape rate and the apoptosis rate were higher in the Longdanxiegan decoction group than in the untreated group. Longdanxiegan decoction significantly down-regulated the expression level of Bcl-2 and up-regulated Bax, and actived caspase in HaCaT cells. The cell apoptosis caused by Longdanxiegan decoction decreased in the cells pretreated by Z-DEVD-FMK (inhibitor of caspase-3). Conclusion: Longdanxiegan decoction can significantly induce apoptosis of human keratinocytes, which may be through down-regulation of Bcl-2 and up-regulation of caspase-3 or Bax.

Key words: Longdanxiegan decoction, HaCaT cells, psoriasis, apoptosis