中国麻风皮肤病杂志 ›› 2021, Vol. 37 ›› Issue (7): 413-418.doi: 10.12144/zgmfskin202107413

• 论著 • 上一篇    下一篇

胎鼠真皮成纤维细胞皮内注射对UV诱导小鼠皮肤光老化的改善作用

邱勰,雷铁池,韩冰玉,姚云竹,廖志凯,胡双海    

  1. 武汉大学人民医院皮肤性病科，湖北武汉，430060
  • 出版日期:2021-07-15 发布日期:2021-06-25
  • 通讯作者: 雷铁池，E-mail: tchlei@whu.edu.cn

Improvement effect of fetal dermal fibroblasts intradermal injection on the photoaged mouse skin induced by UV

QIU Xie, LEI Tiechi, HAN Bingyu, YAO Yunzhu, LIAO Zhikai, HU Shuanghai   

  1. Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan 430060, China
  • Online:2021-07-15 Published:2021-06-25
  • Contact: LEI Tiechi, E-mail: tchlei@whu.edu.cn

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摘要： 目的：探索体外培养胎鼠真皮成纤维细胞（fibroblasts，FBs）皮内注射对小鼠背部光老化皮肤的改善作用。方法：分离培养10只第18.5天（E18.5）BALB/c胎鼠皮肤，体外培养FBs两天；用CM-Dil荧光染料进行活细胞标记，以示FBs注射后在体内存活情况。取BALB/c雄性小鼠20只，随机分为：未处理组5只、模型组5只（紫外线照射）、空白组5只（紫外线照射+注射PBS）、治疗组5只（紫外线照射+注射FBs），通过真表皮厚度及I型前胶原羧基端肽（PICP）的表达量评估胎鼠FBs对皮肤老化的改善作用。结果：胎鼠FBs形态多为梭型或不规则三角形，中央有卵圆形的细胞核，CM-Dil 细胞示踪试验显示培养细胞皮内注射后第3天活性最强。治疗组小鼠真皮厚度（243.659±6.214）μm较空白组（126.198±12.352）μm明显增厚，差异有统计学意义（P<0.05）。治疗组PICP浓度（120.357±10.260）ng/mL高于空白组（95.357±13.471）ng/mL，差异有统计学意义（P<0.05）。结论：胎鼠FBs皮内注射可改善紫外线诱导的小鼠皮肤老化。

关键词: 成纤维细胞, 皮肤老化, 皮内注射, 细胞疗法

Abstract: Objective: To investigate the effect of intradermal injection of fetal dermal fibroblasts (FBS) cultured in vitro on the photoaged dorsal skin of mice. Methods: The dermal fibroblasts were isolated and cultured from 10 BALB/c mouse embryo skins（E18.5）in vitro for two days. The living cells were labeled with CM-Dil dye to track the survival of FBs at the injection site. Twenty BALB/C male mice were randomly divided into 4 groups with 5 mice in each group: untreated group, model group (treated with ultraviolet), blank group (treated with ultraviolet +PBs injection) and treatment group (treated with ultraviolet +FBs). The effect of fetal FBs on skin photoaging was evaluated by the thickness of dermis and epidermis and the expression level of procollagen type IC-terminal propeptide (PICP). Results:  FBS of fetal mice were mostly shuttle type or irregular triangle, with oval nucleus in the center. The number of CM-Dil-labeled cells was largest on the 3rd day post-injection. The dermis thickness of the treatment group was significantly thicker than that of the blank group(243.659±6.214μm vs.126.198±12.352μm) (P<0.05). The concentration of PICP in the treatment group (120.357±10.260) ng/mL was higher than that in the blank group (95.357±13.471) ng/mL with a significant difference (P<0.05). Conclusion: Intradermal injection of fetal FBS can improve the skin photoaging induced by ultraviolet in mice.

Key words: fibroblasts, skin aging, intradermal injection, cell therapy