中国麻风皮肤病杂志 ›› 2022, Vol. 38 ›› Issue (6): 349-354.doi: 10.12144/zgmfskin202206349

• 论著 • 上一篇    下一篇

Clascoterone对SZ95人皮脂腺细胞增殖、脂质合成和炎症因子表达的影响

叶枫1,莫小辉1,胡婷婷1,李昕1,魏子妤1,李嘉祺1,陈广洁2,Christos C. Zouboulis3,鞠强1   

  1. 1上海交通大学医学院附属仁济医院皮肤科，上海，200127; 2上海交通大学医学院免疫学与微生物学系，上海，200025;3勃兰登堡医学院德绍临床医学中心皮肤性病科、变态反应及免疫科，德国德绍，06847
  • 出版日期:2022-06-15 发布日期:2022-04-14
  • 通讯作者: 鞠强，E-mail: qiangju@aliyun.com

Effect of clascoterone on the proliferation, lipid synthesis and inflammatory factors expression of human SZ95 sebocytes in vitro

YE Feng1, MO Xiaohui1, HU Tingting1, LI Xin1, WEI Ziyu1, LI Jiaqi1, CHEN Guangjie2, Christos C. Zouboulis3, JU Qiang1   

  1. 1 Department of Dermatology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China; 
    2 Department of Immunology and Microbiology, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025, China; 
    3 Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodore Fontane, Dessau 06847, Germany
  • Online:2022-06-15 Published:2022-04-14
  • Contact: JU Qiang, E-mail: qiangju@aliyun.com

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摘要： 目的：明确clascoterone对雄激素作用下人皮脂腺细胞增殖、脂质合成和炎症因子表达的影响，探索其抗痤疮机制。方法：体外培养SZ95人皮脂腺细胞，分为双氢睾酮（DHT）组、clascoterone组，clascoterone+DHT组，CCK8法检测细胞增殖，尼罗红染色检测细胞内中性脂质，蛋白免疫印迹检测脂质合成相关基因表达。酶联免疫吸附检测细胞培养上清液中炎症因子蛋白，RT-qPCR检测细胞因子转录水平。结果：与DHT组相比，clascoterone和DHT共培养24h后细胞数量出现显著下降（P＜0.01）；在亚油酸（LA）作用下，clascoterone+DHT组细胞内中性脂质低于DHT组（P＜0.0001）；此外，clascoterone+DHT组PPARγ、PLIN2、FASN、SCD1等脂质合成相关基因的表达、炎症因子IL-1α、IL-1β、IL-6、IL-8及相应蛋白水平低于DHT组(均P<0.05)。结论：clascoterone体外对人皮脂腺细胞增殖、脂质合成相关基因和炎症因子具有调节作用，可能是其抗痤疮治疗机制之一。

关键词: clascoterone, 皮脂腺细胞, 寻常痤疮

Abstract: Objective: To determine the effect of clascoterone on androgen-induced human sebocytes proliferation, lipid synthesis and inflammatory factors expression in vitro and explore its anti-acne mechanism. Methods: The immortalized human sebocytes SZ95 were cultrured in vitro and then divided into Dihydrotestosterone (DHT) group, clascoterone group and clascoterone+DHT group. The cell proliferation, intracellular neutral lipids, lipid synthesis-related proteins expression, inflammatory factors and transcription level of cytokines was detected by CCK8, nile red staining and western blot, enzyme-linked immunosorbent assay and RT-qPCR respectively. Results: Compared with the DHT group, the number of cells in the clascoterone+DHT group significantly decreased (P<0.01) after 24-hour treatment. After treated with linoleic acid (LA), the level of neutral lipids in the clascoterone+DHT group was significantly  lower than that in the DHT group (P<0.0001). The lipid synthesis-related proteins such as PPARγ, PLIN2, FASN and SCD1, the transcription levels of IL-1α, IL-1β, IL-6, IL-8 and the corresponding protein levels in the clascoterone+DHT group was significantly  lower than those in the DHT group (Ps<0.05) . Conclusion: Clascoterone has a partly androgen-independent regulatory effect on human sebocytes proliferation, lipid synthesis-related genes and inflammatory factors in vitro, which might be one of anti-acne mechanisms.

Key words: clascoterone, sebocytes, acne vulgaris