中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (2): 77-81.

• 论著 • 上一篇    下一篇

D-半乳糖诱导性亚急性衰老大鼠模型皮肤胶原代谢及相关通路分子的表达

纪秀军1 闫丙健2 赵彩玲2   

  1. 1山东省莱芜市皮肤病防治所， 山东莱芜， 271100 2 山东省莱芜市人民医院，山东莱芜，271100
  • 出版日期:2016-02-15 发布日期:2018-12-18

Expression of collagen metabolism and the related signaling pathway in skin aging rat models induced by D-Galactose

JI Xiujun1*，YAN Bingjian2，ZHAO Cailing2   

  1. 1. Laiwu Provincial Institute of Dermatology, Laiwu 271100, China 2. Laiwu People’s Hospital, Laiwu 271100, China
  • Online:2016-02-15 Published:2018-12-18

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摘要： 目的：明确D-半乳糖诱导的亚急性衰老大鼠皮肤胶原代谢及TGFβ1/smads通道分子的表达。方法：20只大鼠随机分为对照组和衰老组，衰老组大鼠颈背部皮下注射D-半乳糖125 mg/kg?d，连续6周，对照组以同样的方法注射相同剂量的无菌生理盐水。活体皮肤共聚焦显微镜观察两组大鼠皮肤胶原纤维密度的变化。RT-PCR检测TGFβ1、Smad3、 I 型前胶原蛋白、III 型前胶原蛋白及脂质金属蛋白酶抑制剂-1的mRNA水平。结果：衰老组大鼠皮肤胶原纤维密度及TGFβ1、Smad3、 I 型前胶原蛋白、脂质金属蛋白酶抑制剂-1mRNA均低于正常对照组(P<0.05).；脂质金属蛋白酶-1mRNA表达量高于对照组(P<0.05).； III 型前胶原蛋白mRNA表达量在两组间没有差异。结论：D-半乳糖可通过下调TGFβ1/Smads通路分子的表达量抑制I 型前胶原蛋白合成，并通过调节脂质金属蛋白酶-1/脂质金属蛋白酶抑制剂-1促进胶原蛋白降解，使皮肤出现亚急性衰老效应。

关键词: D-半乳糖, 大鼠衰老模型, 胶原代谢, TGFβ1/smads通路

Abstract: Objective: To determine the expression of collagen metabolism and the related signaling pathway in skin aging rat models induced by D-Galactose. Methods: Twenty rats were randomly divided into the D-gal group and control group. Ten rats in the D-gal group were given subcutaneous injection of D-galactose，125mg/kg?d for 6 weeks. The rats in the control group were treated with the same volume of normal saline. The collagen density was detected by the confocal microscopy. The expression of ColⅠ, ColⅢ, MMP-1, TIMP-1, TGFβ1 and Smad3 mRNA was detected by RT-PCR. Results: The collagen density of rats and the level of ColⅠ, MMP-1, TIMP-1, TGFβ1 and Smad3 mRNA were lower in the D-gal group than in the control group (P<0.05). The expression of MMP-1mRNA was higher in the D-gal group than in the control group. There was no significant difference in the level of ColⅢ between the D-gal group and control group. Conclusion: D-Galactose inhibits the ColⅠ synthesis through down-regulating the expression of TGFβ1/Smad3 and accelerates the degradation of collagen through regulating the balance between MMP-1 and TIMP-1, which makes the skin into subacute aging.

Key words: D-Galactose, rat skin aging model, collagen metabolism, TGFβ1/smads pathway