中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (5): 270-274.

• 论著 • 上一篇    下一篇

麻风患者血清Th1/Th2/Th17相关细胞因子检测

李建可1,2 王 川2 李富荣2 初同胜2 刘殿昌2 于修路2 刘 红2 张福仁2   

  1. 1 济南大学 山东省医学科学院 医学与生命科学学院, 济南, 250062 2 山东省皮肤病性病防治研究所, 济南, 250022
  • 出版日期:2016-05-15 发布日期:2018-12-24
  • 通讯作者: 张福仁，E-mail:zhangfuren@hotmail.com

Detection of serum Th1, Th2 and Th17 related cytokines in the patients with leprosy

LI Jianke1,2, WANG Chuan2, LIU Hong2, ZHANG Furen2.   

  1. 1.School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250062 ,China 2. Shandong Provincial Institute of Dermatology and Venereology, Jinian 250022, China
  • Online:2016-05-15 Published:2018-12-24
  • Contact: E-mail:zhangfuren@hotmail.com

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摘要： 目的：检测麻风患者血清中Th1/Th2/Th17相关细胞因子水平，探讨其在麻风发病中的作用。方法：利用微量样本多重蛋白定量技术检测12例新发麻风患者，29例治愈麻风患者及37例正常人血清中IL-1、IL-2、IL-4、IL-6、IL-10、TNF、IFN-γ和IL-17A水平。利用Kruskal-Wallis和Nemenyi方法进行组间比较。结果：新发组患者血清IL-1和IL-2水平显著高于治愈组（P≤0.005）；新发组及治愈组患者血清IL-6水平均显著高于对照组（P＜0.005）；新发组患者血清IFN-γ 和IL-17A水平显著高于对照组（P＜0.005）。新发组、治愈组和对照组IL-4、IL-10和TNF浓度差异无统计学意义。结论：Th1/Th2/Th17某些相关细胞因子可能与麻风的发病有关。

关键词: 麻风, 细胞因子, 微量样本多重蛋白定量技术

Abstract: Objective: To detect the levels of Th1, Th2 and Th17 related cytokines in the patients with leprosy and to explore the role of those cytokines in leprosy. Methods: The serum levels of IL-1, IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ and IL-17A were detected by flow cytometric bead array in 12 newly reported leprosy patients, 29 cured leprosy patients and 37 healthy controls. The differences between groups were compared by Kruskal-Wallis test and Nemenyi test. Results: The levels of IL-1 and IL-2 in newly reported leprosy patients were higher than that in cured leprosy patients, with significant difference (P＜0.005). The levels of IL-6 in newly reported leprosy patients and cured leprosy people were higher than those in healthy controls (P＜0.005). The levels of IFN-γ and IL-17A in newly reported leprosy patients were higher than those in healthy controls (P＜0.005). There was no difference in the levels of IL-4，IL-10 and TNF among newly reported leprosy patients, cured leprosy patients and healthy controls. Conclusion: Th1, Th2 and Th17 related cytokines may be associated with the development of leprosy.

Key words: leprosy, cytokines, flow cytometric bead array