中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (6): 335-338.

• 论著 • 上一篇    下一篇

抗白灵霜在黑素合成中对TYR和MITF的调节作用

徐漫远1 孔迎辉1 闵仲生2   

  1. 1南京医科大学附属淮安第一医院，淮安，223300；2南京中医药大学附属江苏省中医院，南京，210029
  • 出版日期:2016-06-15 发布日期:2018-12-27
  • 通讯作者: 闵仲生 E-mail：minzhsh@sinan.com.

The regulative effect of Kang Bai Ling cream on TYR and MITF in melanogenesis

XU Manyuan, KONG Yinghui, MIN Zhongsheng.   

  1. 1.Huai'an First People's Hospital Affiliated Nanjing Medical University, Huai’an 223300, China； 2.Nanjing Province Hospital of TCM Affiliated Hospital of Nanjing University of TCM, Nanjing 210029, China
  • Online:2016-06-15 Published:2018-12-27
  • Contact: MIN Zhongsheng, E-mail:minzhsh@sin.com

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摘要： 目的：明确抗白灵霜(KBL)在黑素合成中对TYR和MITF的调节作用。方法：体外培养小鼠B16黑素瘤细胞，分为空白对照组和不同浓度KBL（5 mg/mL，10mg/mL和15mg/mL）用NaOH溶解法检测黑素合成量，Real time-PCR检测TYR和MITF基因mRNA表达，Western Blot检测KBL组TYR和MITF蛋白的表达。结果：各浓度KBL组黑素合成量及TYR和MITF基因的mRNA和蛋白表达的水平均高于空白对照组，且呈浓度依赖性。结论：KBL可能通过上调MITF基因的表达，激活参与黑素合成的TYR以促黑素合成。

关键词: 抗白灵霜, 小鼠B16黑素瘤细胞, 酪氨酸酶, 小眼畸形相关转录因子, 黑素合成

Abstract: Objective: To determine the regulative effect of Kang Bai Ling (KBL) creams on TYR and MITF in melanogenesis. Method: The cultured B-16 murine melanoma cells were divided into blank group and KBL groups with different concentration (5 mg/mL, 10mg/mL and 15mg/mL). The amount of melanin was detected by NaOH dissolved method. The expression of TYR and MITF mRNA and protein was detected by real time-PCR and Western blot respectively. Result: The amount of melanin, the expressions of TYR and MITF mRNA and protein in KBL groups were higher than those in blank group. The effects were significant in a concentration dependent manner. Conclusions: KBL can promote melanogenesis through up-regulating the expression of MITF genes and activating TYR.

Key words: KBL, B16 murine melanoma cells, tyrsionase, MITF, melanogenesis