中国麻风皮肤病杂志 ›› 2021, Vol. 37 ›› Issue (10): 625-628.doi: 10.12144/zgmfskin202110625

• 论著 • 上一篇    下一篇

NB-UVB对黑素细胞黑素合成相关基因表达的影响

孙晶莹,封青,李研,霍雪萍,孙丽君   

  1. 陕西省人民医院中心实验室，陕西西安，710068
  • 出版日期:2021-10-25 发布日期:2021-08-31
  • 通讯作者: 孙丽君，E-mail: 45745642@qq.com

Effect of NB-UVB on expression of genes related to melanin synthesis in melanocytes

SUN Jingying, FENG Qing, LI Yan, HUO Xueping, SUN Lijun   

  1. Department of Dermatology, Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an 710068, China
  • Online:2021-10-25 Published:2021-08-31
  • Contact: SUN Lijun, E-mail: 45745642@qq.com

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摘要： 目的：研究NB-UVB照射对黑素细胞的细胞活性、黑素合成、黑素合成相关基因表达的影响。方法：培养黑素细胞系PIG1，利用MTT法检测不同剂量NB-UVB（400、800、1200、1600、2000 mJ/cm2）照射后细胞的增殖率，采用NaOH裂解法测定细胞中的黑素含量，RT-PCR和Western blot方法检测UVB照射后12个黑素合成相关基因Tyr、Tyrp1、Tyrp2、OA1、MART-1、Pmel17、VAT-1、OCSP、syntenin、CHCHD3、flotillin-1、GPNMB的mRNA和蛋白的表达。结果：NB-UVB照射剂量在400、800、1200 mJ/cm2 时，细胞活力无明显变化，照射剂量为1600、2000 mJ/cm2时细胞活力明显下降。NB-UVB照射剂量为400、800、1200 mJ/cm2 时，随着照射剂量的增加，黑素的合成增加；NB-UVB照射后，12种黑素体蛋白中，Tyr、Tyrp2、GPNMB、Syntenin、MART-1的基因mRNA及蛋白表达显著升高。结论：UVB照射显著增加了黑素合成和相关基因的表达。

关键词: 窄谱中波紫外线, 黑素细胞, 黑素合成相关基因

Abstract: Objective: To study the effects of NB-UVB irradiation on the melanocytes viability, melanin synthesis, and melanin synthesis-related gene expression. Methods: The melanocyte cell line PIG1 was cultured. The cell proliferation after different doses of UVB irradiation (400, 800, 1200, 1600, 2000 mJ/cm2) was detected by MTT method. The melanin content was detected by NaOH lysis method. The expression of melanin synthesis-related genes, Tyr, Tyrp1, Tyrp2, OA1, MART-1, Pmel17, VAT-1, OCSP, syntenin, CHCHD3, flotillin-1, GPNMB after UVB irradiation were detected by RT-PCR and Western blot. Results: The cell viability decreased significantly after irradiated by NB-UVB radiation at dose of 1600 and 2000 mJ/cm2. There was no significant change of cell viability after irradiated by NB-UVB radiation at dose of 400, 800 and 1200 mJ/cm2, and melanin synthesis increased with increasing radiation dose. After ultraviolet irradiation, of the 12 melanin synthesis-related genes, 5 of Tyr, Tyrp2, GPNMB, Syntenin, MART-1 mRNA and protein were significantly increased. Conclusion: NB-UVB irradiation significantly increased the melanin synthesis and the expression of related genes.

Key words: NB-UVB, melanocytes, melanin synthesis related genes