中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (1): 16-19.doi: 10.12144/zgmfskin202001016

• 论著 • 上一篇    下一篇

UVB下调IFN-γ诱导的正常人角质形成细胞表面抗原的表达

朱健伟1,骆丹2   

  1. 1浙江医院皮肤科，浙江杭州，310013；2南京医科大学第一附属医院皮肤科，江苏南京，210029
  • 出版日期:2020-01-15 发布日期:2020-03-16
  • 通讯作者: 朱健伟，E-mail：zjwmed@163.com

Down-regulation of IFN-γ-induced over-expression of surface antigens on normal human keratinocytes by UVB

ZHU Jianwei1, LUO Dan2   

  1. 1 Department of Dermatology, Zhejiang Hospital, Hangzhou 310013, China; 
    2 Department of Dermatology, The First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China
  • Online:2020-01-15 Published:2020-03-16
  • Contact: ZHU Jianwei, E-mail: zjwmed@163.com

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摘要： 目的：研究中波紫外线（UVB）对IFN-γ体外诱导的正常人角质形成细胞(KC)表面抗原表达的影响及可能机制。方法：分离培养原代人KC，用适当浓度的IFN-γ对细胞进行预处理，再用生理剂量的UVB照射细胞。流式细胞术测定照射前后细胞表面CD40、CD54、MHC-II的表达，放射免疫法测定照射前后细胞上清液中α-MSH浓度，ELISA测定上清液中IL-6、IL-8、TNF-α等细胞因子浓度。结果：KC低水平表达CD40、CD54、MHC-II抗原，IFN-γ处理后，细胞活性增强，表面抗原的表达显著增加，再用UVB照射后，细胞活性下降，表面抗原表达明显下调，而上清液中α-MSH的浓度则逐渐上升，照后72 h达最高峰。KC经可溶性CD40配体(sCD40L)处理后，IL-8的释放和CD54的表达加强。结论：UVB能抑制IFN-γ诱导的KC表面抗原的表达，这可能与UVB诱导KC自分泌产生α-MSH等免疫抑制物有关。

关键词: 角质形成细胞, 表面抗原, 干扰素-γ, 中波紫外线, α-黑素细胞刺激素

Abstract: Objective: To investigate the effect of UVB irradiation on the expression of surface antigens induced by IFN-γ on normal human keratinocytes in vitro. Methods: A routine culture of keratinocytes was established which were pre-incubated with recombinant human IFN-γ, and then, irradiated with UVB. The expression of surface antigens, such as CD40, CD54, MHC-II, was detected by FACS and the level of α-MSH before and after UVB irradiation was detected by RIA. The levels of cytokines, such as IL-6, IL-8 and TNF-α, were detected by ELISA. Results: The levels of surface antigens were low, which significantly increased after stimulated by IFN-γ, after that, markedly decreased after UVB irradiation but the level of α-MSH increased to peak at 72 h after irradiation. The levels of CD54 and IL-8 release expression were increased after treated by sCD40L. Conclusion: UVB irradiation down-regulates the level of surface antigens induced by IFN-γ on keratinocytes, which may be related with α-MSH induced by UVB. 

Key words: keratinocytes, surface antigens, IFN-γ, UVB, α-MSH