中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (10): 591-594.doi: 10.12144/zgmfskin202010591

• 论著 • 上一篇    下一篇

NB-UVB对白癜风患者黑素细胞生物学特性的影响

袁相凤,罗增香,刘相明,孟慧娟,李建娜,刘国艳   

  1. 潍坊医学院附属医院皮肤科，潍坊，261013
  • 出版日期:2020-10-15 发布日期:2020-09-22
  • 通讯作者: 刘国艳，E-mail: wfliuguoyan@126.com

Effect of NB-UVB on biological characteristics of melanocytes from vitiligo lesions

YUAN Xiangfeng, LUO Zengxiang, LIU Xiangming, MENG Huijuan, LI Jianna, LIU Guoyan   

  1. Department of Dermatology, Affiliated Hospital of Weifang Medical University, Weifang 261013, China
  • Online:2020-10-15 Published:2020-09-22
  • Contact: LIU Guoyan, E-mail: wfliuguoyan@126.com

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摘要： 目的：明确窄谱中波紫外线(narrow-band UVB, NB-UVB)对白癜风患者的黑素细胞生物学特性的影响。方法：提取34例白癜风患者皮损处黑素细胞进行体外培养。每例样本分组后分别接受不同的照射剂量（0、20、40、60、80、100 mJ/cm2）的NB-UVB处理，照射后1 d采用免疫印迹法对黑素细胞中微管相关蛋白轻链3 II/I(LC3 II/I)、酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)、自噬信号磷酸化磷酸腺苷蛋白激酶(p-AMPK)、P26蛋白的表达情况进行测定。照射后2d、3d、4d采用MTT法对黑素细胞的增殖活性进行测定，采用NaOH染色法对黑素细胞中黑素含量进行测定。结果：随着NB-UVB照射剂量的增加,LC3 II/I和p-AMPK表达水平逐渐上调，p-mTOR和P26的表达水平逐渐下降。不同NB-UVB剂量下，黑素细胞增殖水平增加，但随剂量的增加，增殖的程度逐渐减弱。不同NB-UVB剂量黑素细胞中黑素水平升高，且呈剂量依赖式递增。结论：NB-UVB能够有效调节白癜风患者黑素细胞自噬相关蛋白表达水平，活化自噬信号通路，促进黑素细胞增殖及黑素合成能力。

关键词: 窄谱中波紫外线, 白癜风, 黑素细胞, 生物学特性

Abstract: Objective:To determine the effect of narrow-band UVB  (NB-UVB)on the biological characteristics of melanocytes from the lesions of patients with vitiligo. Methods: Melanocytes from the skin lesions of 34 patients with vitiligo were cultured in vitro. Then the melanocytes were divided into 6 groups with different  radiation doses (0, 20, 40, 60, 80, 100 mJ/cm2). The levels of microtubule associated protein light chain 3 II/I (LC3 II/I), acidified mammalian rapamycin target protein (p-mTOR), autophagy signal phosphorylated adenosine monophosphate protein kinase (p-AMPK) and P26 protein in melanocytes were detected by Western blot at 1 day after radiation. The proliferation activity of melanocytes was measured by MTT at 2 day, 3 day and 4 day after radiation. The content of melanin in melanocytes was determined by NaOH staining method. Results: The expression levels of LC3 II/I and p-AMPK increased after NB-UVB treatment in a dose-dependent manner, while the expression levels of p-mTOR and P26 decreased. The proliferation activity of melanocytes increased after treated by NB-UVB, but the degree of increase reduced gradually with the increasing doses of NB-UVB. The level of melanin increased in a dose-dependent manner. Conclusion: NB-UVB can effectively regulate the expression of autophagy-related proteins in melanocytes and activate autophagy signal pathways. At the same time, it can promote the proliferation of melanocytes and melanin.

Key words: NB-UVB, vitiligo, melanocytes, biological characteristics