中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (12): 705-708.

• 论著 •    下一篇

RGS4表达上调对人恶性黑素瘤细胞的M14增殖和迁移的影响

薛晓彤1,2  焦敬3  张倩倩3  黄淑红1  党宁宁2   

  1. 1山东大学齐鲁医学院，山东济南，250012
    2山东大学附属济南市中心医院皮肤科，山东济南，250013
    3中国人民解放军第88医院，山东泰安，271000
  • 出版日期:2017-12-15 发布日期:2018-12-12
  • 通讯作者: 党宁宁，E-mail：15318816250@163.com

Up-regulation of RGS4 inhibits the proliferation and migration of human malignant melanoma cells in vitro

XUE  Xiaotong1,2, JIAO jing3, ZHANG qianqian3, HUANG Shuhong1, DANG Ningning2.   

  1. 1. School of Medicine, Shandong University, Jinan 250012,China；
    2. Department of Dermatology, Jinan Central Hospital affiliated to Shandong University, Jinan 250013,China；
    3. Department of Dermatology,The Chinese People's Liberation Army 88 Hospital, Taian 271000, China
  • Online:2017-12-15 Published:2018-12-12
  • Contact: DANG Ningning，E-mail: 15318816250@163.com

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摘要： 目的：明确体外RGS4基因表达上调对恶性黑素瘤M14细胞中增殖及迁移的影响。方法：体外培养M14细胞，分为对照组（转染pcDNA3.1空质粒）和实验组（转染pcDNA3.1-RGS4质粒）。RT-PCR和Western blot检测转染后RGS4 mRNA和RGS4蛋白水平的表达，CCK-8和克隆形成实验检测RGS4上调对恶性黑素瘤M14细胞增殖的影响，划痕实验检测RGS4高表达对M14细胞迁移的影响。结果：实验组细胞中RGS4 mRNA表达量是对照组的3.25×103倍（P<0.05），蛋白表达量是对照组的6.73倍（P<0.05）。实验组M14细胞OD值为1.33±0.07，低于对照组的1.61±0.11（P<0.05），实验组M14细胞克隆数为34±5.53，低于对照组的56±7.68（P<0.05）。实验组M14细胞迁移能力低于对照组。结论：RGS4高表达能够抑制体外恶性黑素瘤细胞的增殖和迁移，RGS4在恶性黑素瘤的发生、发展中可能发挥重要作用。

关键词: G蛋白偶联受体, RGS4基因, 恶性黑素瘤, 增殖和迁移

Abstract: Objective: To determine the influence of pcDNA3.1-RGS4 vector up-regulating RGS4 expression on the proliferation and migration of human malignant melanoma cells in vitro. Methods: The cells were cultured in vitro, then divided into the control group (pcDNA3.1) and experimental group (pcDNA3.1- RGS4). The expression of RGS4 mRNA and protein in M14 cell was detected by RT-PCR and Western blot. The proliferation of M14 cell was detected by CCK-8 and colon formation assay. The migration of M14 cell was detected by wound healing assay. Results: The levels of RGS4 mRNA and protein in the experimental group were 3.25×103 and 6.73 times more than that in the control group (Ps<0.05). The OD scores and clone number of M14 cells in the experimental group were 1.33±0.07 and 34±5.53, which was lower than that in the control group (1.61±0.11 and 56±7.68) (Ps<0.05). M14 cellular migration ability in the experimental group was weaker than that in the control group. Conclusion: RGS4 up-regulation inhibits the M14 cell proliferation and migration in malignant melanoma. RGS4 may play an important role in the development of malignant melanoma.

Key words: GPCR, RGS4 gene, malignant melanoma, proliferation and migration