中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (1): 4-7.

• 论著 • 上一篇    下一篇

上转换发光技术侧流免疫夹心法检测59例麻风患者和87例家庭接触者PGL-I抗体结果分析

张信辉1，杨梨丽1，杨 琴2，赵廷芳3，杨昌明3，戴 乾4，元康4，翁小满5，温 艳5，Anouk. van Hooij6, Paul Corstjens6 , Annemieke Geluk6，赖雅芳7，付云7，李进岚1   

  1. 1、贵州省疾病预防控制中心（贵阳 550004） 2、贵阳市疾病预防控制中心（贵阳 550002） 3、黔西南州疾病预防控制中心（兴义 562400） 4、安顺市疾病预防控制中心（安顺 561000） 5、首都医科大学附属北京友谊医院（北京 100050） 6、荷兰莱顿大学医学中心传染病研究所 7、贵州医科大学（贵阳 550004）
  • 出版日期:2018-01-15 发布日期:2018-12-04
  • 通讯作者: 李进岚，E-mail:740820442@qq.com
  • 基金资助:
    1、贵州省卫生计生委科学技术基金项目（gzwjkj2014-1-078） 2、国家自然科学基金（编号：81260436） 3、荷兰莱顿大学医学中心传染病研究所麻风病合作项目（MALTALEP）

Detection of PGL-I antibody in 59 patients with leprosy and 87 household contacts by lateral flow immuno-sandwich assay using up-converting phosphor reporter particles

ZHANG Xinhui1,YANG Lili1, YANG Qin2, ZHAO Tingfang3, YANG Changming3, DAI Qian4, YUAN Kang4, WENG Xiaoman5, WEN Yan5, Anouk.van Hooij6, Paul Corstjens6 , Annemieke Geluk6, LAN Yafang7, FU Yun7, LI Jinlan1.   

  1. 1、Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China； 2、Guiyang Center for Disease Control and Prevention,Guiyang, Guizhou 550002, China； 3、Qianxinan Center for Disease Control and Prevention, Xingyi, Guizhou 562400, China； 4、Anshun Center for Disease Control and Prevention,Anshun, Guizhou 561000, China； 5、Beijing Friendship Hospital affiliated to Capital Medical University, Beijing 100050, China； 6、Institute of Infectious Diseases, Leiden University Medical Center, Holland; 7.Guizhou Medical University, Guiyang, Guizhou 550002 , China
  • Online:2018-01-15 Published:2018-12-04
  • Contact: LI Jinlan，E-mail:740820442@qq.com

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摘要： 目的：评估上转换发光技术侧流免疫夹心法（UCP-LFA）检测PGL-I抗体对麻风接触者发病风险预测的价值。方法：采用UCP-LFA 方法检测贵州59例麻风患者、87例家庭接触者和55例健康对照进行PGL-I抗体水平。数据统计采用SPSS 20.0进行卡方分析。根据ROC曲线确定临界值。结果：麻风患者中PGL-I抗体阳性率为81.4%、麻风接触者为5.7%、健康对照人群为1.8%,三组人群PGL-I抗体阳性率差异有统计学意义（χ2 =126.47，P<0.005）。少菌型和多菌型PGL-I抗体阳性率分别为40% 和89%，差异有统计学意义（χ2=13.386，P<0.005）。BI+、BI-患者PGL-I抗体阳性率分别为83%和0%，差异有统计学意义（χ2 =17.560，P<0.005）。结论：麻风患者PGL-1抗体阳性率>麻风病接触者>正常对照，多菌型PGL-1抗体阳性率高于少菌型，提示PGL-I IgM抗体水平对麻风发病有一定的预测作用。

关键词: 上转换发光技术侧流免疫夹心法, 麻风

Abstract: Objective: To evaluate the value of lateral flow immuno-sandwich assay using up-converting phosphor reporter particles (UCP-LFA) in predicting the risk of leprosy contacts by detecting PGL-I antibody. Methods: The PGL-I IgM level were measured by UCP-LFA in 59 patients with leprosy, 87 household contacts and 55 healthy controls in Guizhou. Data statistics were analyzed by using SPSS 20.0 for chi-square test. The?critical value was identified through receiver operating characteristic curve. Results: The positivity rate of anti-PGL-I IgM in patients, contacts and healthy controls were 81.4%, 5.7% and 1.8%, with significant differences between them (χ2 =126.47，P<0.005). The positivity rates of anti-PGL-I IgM in patients with paucibacillary leprosy (PB) and multibacillary (MB) were 40% and 89%, with a significant difference (χ2=13.386，P<0.005).The positivity rates of anti-PGL-I IgM in patients with BI+ and BI- were 83% and 0%, with a significant difference (χ2 =17.560，P<0.005). Conclusion: The PGL-1 IgM antibody can predict the risk of leprosy.

Key words: UCP-LFA, leprosy