中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (2): 83-86.

• 论著 • 上一篇    下一篇

苯并芘与肽聚糖体外协同对人皮脂腺细胞炎症因子表达的影响

侯霄枭1，曹 珂1，胡婷婷1，潘展砚1，Christos C. Zouboulis2，鞠 强1   

  1. 1 上海交通大学医学院附属仁济医院皮肤科，上海，200127 2 勃兰登医学院德绍临床医学中心皮肤病与性病科，变态反应及免疫科，德国 德绍，06847
  • 出版日期:2018-02-15 发布日期:2018-12-06
  • 通讯作者: 鞠强，E－mail: qiangju@aliyun.com
  • 基金资助:
    国家自然科学基金(编号:81472894)

Effect of Bap and PGN on the expression of inflammatory cytokines in human SZ95 sebocytes in vitro

HOU Xiaoxiao1, CAO Ke1, HU Tingting1, PAN Zhanyan1, Christos C. Zouboulis2，JU Qiang1.   

  1. 1.Department of Dermatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China; 2 Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodore Fontane, Dessau, Germany
  • Online:2018-02-15 Published:2018-12-06
  • Contact: JU Qiang, E-mail: qiangju@aliyun.com

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摘要： 目的：检测苯并芘（Bap）和革兰氏阳性菌细菌壁肽聚糖（PGN)体外对SZ95人皮脂腺细胞炎症因子表达的影响。方法：体外培养SZ95永生化人皮脂腺细胞，分为空白对照组、Bap组、PGN组和（Bap+PGN）组， PGN浓度为20μg/mL，Bap浓度 为10-5mol/L。 RT-PCR和ELISA检测SZ95永生化人皮脂腺细胞分泌白介素(IL-1α、IL-1β）、TNF-α mRNA和蛋白表达水平。结果：（Bap+PGN）组IL-1α，IL-1β和TNF-αmRNA及蛋白表达水平高于空白对照组、Bap组和PGN组，差异具有统计学意义（P<0.01）。结论：苯并芘协同增强PGN诱导的皮脂腺细胞炎症因子IL-1α，IL-1β，TNF-α的表达，这可解释为什么环境污染可加重或诱导痤疮 的发生。

关键词: 苯并芘, 肽聚糖, 人皮脂腺细胞, IL-1α, IL-1β, TNF-α

Abstract: Objective: To determine the effect of environmental pollutants benzopyrene (Bap) and gram positive bacteria bacterial wall peptidoglycan (PGN) on the expression of inflammatory cytokines (IL-1α, IL-1βa and TNF-α) induced by typical in human SZ95 sebocytes in vitro. Methods: SZ95 sebocytes were cultured and divided into the blank control group, PNG group, Bap group and PNG+ Bap group. The concentration of PNG and Bap was 20μg/mL and 10-5mol/L. The mRNA and protein levels of the inflammatory cytokines (IL-1α, IL-1βa and TNF-α) were detected by RT-PCR and ELISA. Results: The levels of IL-1α, IL-1β and TNF-α in the Bap+PGN group were higher than those in other two groups, with significant differences (P<0.01). Conclusions: Bap synergistic and PGN induce the expression of sebocytes inflammatory cytokines IL-1α, IL-1β and TNF-α, which may explain why environmental pollution can aggravate and induce acne.

Key words: Bap, PGN, human SZ95 sebocytes, IL-1α, IL-1β, TNF-α