中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (5): 261-264.

• 论著 • 上一篇    下一篇

烟曲霉对人THP-1细胞IL-6分泌和信号分子IκBɑ活化的影响

童建波1 杜蕾蕾1 曾 荣1 王丽玮1 刘宇甄1 段志敏1 陈 青2 李 岷1   

  1. 1.中国医学科学院&北京协和医学院皮肤病研究所 江苏省皮肤性病学分子生物学重点实验室，南京 210042；2.江苏省血液中心, 南京 210000
  • 出版日期:2018-05-15 发布日期:2018-12-10
  • 通讯作者: 李岷,E-mail：drlimin@sina.cn；陈青,E-mail：chenqing90@yahoo.com

Effect of Aspergillus fumigatus on the production of interleukin-6 and activation of IκBɑ in human THP-1 monocytes

TONG Jianbo, DU Leilei, ZENG Rong, WANG Liwei,LIU Yuzhen, DUAN Zhimin,CHEN Qing, LI Min   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases, Nanjing 210042, China
  • Online:2018-05-15 Published:2018-12-10
  • Contact: LI Min, E-mail: drlimi CHEN Qing, E-mail: chenqing90@yahoo.comn@sina.cn;

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摘要： 目的: 明确烟曲霉对THP-1细胞中IL-6水平和细胞内信号分子NF-κB抑制蛋白(IκBɑ)激活的影响。 方法：体外培养THP-1细胞，分为106 CFU/mL和107CFU/mL烟曲霉组，β-Glucan (100g/L)阳性对照组，空白对照组， RT-PCR和ELISA检测各组IL-6 mRNA和蛋白表达水平。免疫印迹法检测IκBɑ和磷酸化IκBɑ水平。结果: 106 CFU/mLIL-6 mRNA水平低于107 CFU/mL烟曲霉组，选择107 CFU/mL烟曲霉组作为实验浓度。107 CFU/mL烟曲霉组IL-6 mRNA和蛋白浓度分别为209.38 ± 25.35和(879.86±32.35) ng/L高于空白对照组的1.19 ±0.36和(10.67±0.17)ng/L，差异均有统计学意义(均P<0.05)。烟曲霉作用于THP-1细胞后磷酸化IκBɑ蛋白水平显著升高。NF-κB信号通路抑制剂BAY 11-7082可显著降低烟曲霉组IL-6 mRNA的 表达。 结论 烟曲霉可能通过激活THP-1细胞中的IκBɑ刺激IL-6分泌。

关键词: 烟曲霉, THP-1细胞系, IL-6, NF-ΚB

Abstract: Objective: To determine the effect of Aspergillus fumigatus on the production of interleukin-6 and activation of IκBɑ in an acute monocytic leukemia cell line THP-1. Methods： THP-l cells were cultured in vitro and divided into 106 CFU/mL and 107 CFU/mL A.fumigatus group, 100 μg/mL β-Glucan control group and blank control group. The level of IL-6 mRNA and protein was detected by RT-PCR and ELISA respectively. The level of IκBɑ and phosphorylated IκBɑ was detected by Western blot. Results：The experimental concentration was selected to be 107 CFU/mL, because the level of IL-6 mRNA in the 106 CFU/mL A.fumigatus group was lower than that in the 107 CFU/mL group. The levels of IL-6 mRNA and protein in the 107 CFU/mL A. fumigatus group were 209.38 ± 25.35 and 879.86±32.35 ng/L, which were higher than that in the blank control group (1.19 ± 0.36 and 10.67±0.17 ng/L) , with significant differences (Ps<0.05). The level of phosphorylated IκBɑ protein was increased significantly when induced by 107 CFU/mL A.fumigatus and was decreased when induced by NF-κB signaling pathway inhibitor of bay 11-7082. Conclusion：A. fumigatus stimulates the secretion of IL-6 in human THP-1 cells through activating IκBɑ.

Key words: Aspergillus fumigatus, THP-1 cells line, NF- κB