中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (7): 407-410，413.

• 论著 • 上一篇    下一篇

茶多酚二元醇脂质体的制备及质量评价

宋 娟 于绪东 张振宇 王 栋   

  1. 大连市皮肤病医院药剂科，大连，116021
  • 出版日期:2018-07-15 发布日期:2018-12-14
  • 通讯作者: 宋娟，E-mail：Sj271205219@163.com

Preparation and quality evaluation of tea polyphenol binary ethosomes

SONG Juan, YU Xudong, ZHANG Zhenyu, WANG Dong   

  1. Department of Pharmacy, Dalian Dermatosis Hospital, Dalian 116021,China
  • Online:2018-07-15 Published:2018-12-14
  • Contact: SONG Juan, E-mail:Sj271205219@163.com

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摘要： 目的: 制备茶多酚二元醇脂质体（TP-EL）并对其质量进行评价。方法: 以包封率作为评价指标进行正交试验筛选最佳处方及制备工艺，并评价二元醇脂质体的质量。结果: 各因素最佳的水平组合为：磷脂浓度为4%、磷脂与胆固醇的比为5:1、药脂比为1:8、搅拌时间为40 min。所制备的脂质体大多数为单室脂质体，平均粒径为 123.0 ± 13.2 nm，多分散性指数(PDI)为 0.15 ± 0.07。TP-EL的Zeta 电位为 -35.2 mV，且包封率达71.82 ± 2.03%。结论: 该制剂制备简便、质量稳定。

关键词: 茶多酚, 二元醇脂质体, 包封率, 稳定性

Abstract: Objective: To prepare tea polyphenol binary ethosomes (TP-EL) and evaluate its quality. Methods: Encapsulation efficiency was taken as evaluation index to optimize the formula and preparation technology of TP-EL. The quality of TP-EL was assessed. Results: The optimal formula and preparation technology were as follows: the concentration of phosppholipids was 4%, the ratio of phospholipids /cholesterol was 5:1, the ratio of TP/phospholipids was 1:8 and the stirring time was 40 min. The results showed that most of the TP-EL was unilamellar structure. The TP-EL had an average size of 123.0 ± 13.2 nm with polymer dispersity index (PDI) of 0.15 ± 0.07. The surface charge of TP-EL was -35.2 mV. Moreover, TP was readily incorporated into ethosomes with encapsulation efficiency of 71.82±2.03%. Conclusion: The preparation technology for TP-EL was simple and stable.

Key words: tea-polyphenol, binary ethosomes, encapsulation efficiency, stability