中国麻风皮肤病杂志 ›› 2021, Vol. 37 ›› Issue (6): 397-401.doi: 10.12144/zgmfskin202106397

• 综述 • 上一篇    下一篇

非结核分枝杆菌的临床检测研究进展

潘晴,暴芳芳,刘红,张福仁   

  1. 山东第一医科大学附属皮肤病医院（山东省皮肤病医院，山东省皮肤病性病防治研究所），济南，250022
  • 出版日期:2021-06-15 发布日期:2021-05-27
  • 通讯作者: 刘红，E-mail: hongyue2519@hotmail.com

Update of the clinical detection of nontuberculosis mycobacterium

PAN Qing, BAO Fangfang, LIU Hong, ZHANG Furen   

  1. Shandong Provincial Hospital for Skin Diseases & Shandong Provincial Institute of Dermatology and Venereology, Shandong First Medical University, Jinan 250022, China
  • Online:2021-06-15 Published:2021-05-27
  • Contact: LIU Hong, E-mail: hongyue2519@hotmail.com

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摘要： 非结核分枝杆菌（NTM）是指除结核分枝杆菌复合群和麻风分枝杆菌之外的其他分枝杆菌。近年来，随着艾滋病的流行、器官移植以及免疫抑制剂应用的增多，NTM感染患病率不断上升。目前，NTM鉴定常用方法有抗酸染色、培养、 PNB（对硝基苯甲酸）生长实验等传统检测法，PCR、qPCR等分子生物学鉴定法，免疫学鉴定和药敏学方法等。本文现将非结核分枝杆菌检测方法的国内外研究进展以及每种方法的特点进行综述，以期为NTM感染性疾病的菌株鉴定提供借鉴。

关键词: 非结核分枝杆菌, 检测与鉴定, PCR

Abstract: Nontuberculosis mycobacterium (NTM) defined as any mycobacterial strain other than Mycobacterium tuberculosis complex and Mycobacterium leprae. In recent years, the detection rate of NTM infection has been increasing year by year with the increasing prevalence of AIDS, organ transplantation and the use of immunosuppressive agents. At present, the common methods for NTM identification include acid-fast staining, culture, PNB (p-nitrobenzoic acid) growth test, PCR, qPCR and other molecular biological identification, immunological identification and drug sensitivity methods. In this paper, the update of non-tuberculous mycobacterium detection methods and the characteristics of each method is reviewed, in order to provide reference for the identification of strains of NTM infectious diseases.

Key words: Non-tuberculosis mycobacterium (NTM), detection and identification, PCR